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PLoS Genet 2016 Apr 21;124:e1006001. doi: 10.1371/journal.pgen.1006001.
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Cooperative Wnt-Nodal Signals Regulate the Patterning of Anterior Neuroectoderm.

Yaguchi J , Takeda N , Inaba K , Yaguchi S .

When early canonical Wnt is experimentally inhibited, sea urchin embryos embody the concept of a Default Model in vivo because most of the ectodermal cell fates are specified as anterior neuroectoderm. Using this model, we describe here how the combination of orthogonally functioning anteroposterior Wnt and dorsoventral Nodal signals and their targeting transcription factors, FoxQ2 and Homeobrain, regulates the precise patterning of normal neuroectoderm, of which serotonergic neurons are differentiated only at the dorsal/lateral edge. Loss-of-function experiments revealed that ventral Nodal is required for suppressing the serotonergic neural fate in the ventral side of the neuroectoderm through the maintenance of foxQ2 and the repression of homeobrain expression. In addition, non-canonical Wnt suppressed homeobrain in the anterior end of the neuroectoderm, where serotonergic neurons are not differentiated. Canonical Wnt, however, suppresses foxQ2 to promote neural differentiation. Therefore, the three-dimensionally complex patterning of the neuroectoderm is created by cooperative signals, which are essential for the formation of primary and secondary body axes during embryogenesis.

PubMed ID: 27101101
PMC ID: PMC4839626
Article link: PLoS Genet

Species referenced: Echinodermata
Genes referenced: bmp2 foxe3l hbn lefty1 LOC100887844 LOC115919910 LOC115923729 LOC578903 mapk9 nodall wnt3 wnt6 wnt7b zfhx4
Morpholinos: bmp2 MO1 foxe3l MO3 hbn MO3 lefty1 MO1 nodall MO5 wnt6 MO5 wnt6 MO6 wnt7b MO3 wnt7b MO4

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References [+] :
Angerer, The evolution of nervous system patterning: insights from sea urchin development. 2011, Pubmed, Echinobase