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Since many cell surface receptors exist in their active form as oligomeric complexes, we have investigated the subunit composition of the biologically active sperm receptor in egg plasma membranes from Strongylocentrotus purpuratus. Electrophoretic analysis of the receptor without prior reduction of disulfide bonds revealed that the surface receptor exists in the form of a disulfide-bonded multimer, estimated to be a tetramer. These findings are in excellent agreement with the fact that the NH2-terminus of the extracellular domain of the sperm receptor is rich in cysteine residues. Studies with cross-linking agents of various length and hydrophobicity suggest that no other major protein is tightly associated with the receptor. Given the multimeric structure of the receptor, we investigated the effect of disulfide bond reduction on its biological activity. Because in quantitative bioassays fertilization was found to be inhibited by treatment of eggs with 5 mM dithiothreitol, we undertook more direct studies of the effect of reduction on properties of the receptor. First, we studied the effect of addition of isolated, pure receptor on fertilization. Whereas the non-reduced, native receptor complex inhibited fertilization in a dose-dependent manner, the reduced and alkylated receptor was inactive. Second, we tested the ability of the isolated receptor to mediate binding of acrosome-reacted sperm to polystyrene beads. Whereas beads coated with native receptor bound sperm, those containing reduced and alkylated receptor did not. Thus, these results demonstrate that the biologically active form of the sea urchin sperm receptor consists only of 350 kD subunits and that these must be linked as a multimer via disulfide bonds to produce a complex that is functional in sperm recognition and binding.
Böni-Schnetzler,
Structural requirements for the transmembrane activation of the insulin receptor kinase.
1986, Pubmed
Böni-Schnetzler,
Structural requirements for the transmembrane activation of the insulin receptor kinase.
1986,
Pubmed
Ciapa,
A rapid change in phosphorylation on tyrosine accompanies fertilization of sea urchin eggs.
1991,
Pubmed
,
Echinobase
Eilertsen,
Identification and characterization of two huge protein components of the brush border cytoskeleton: evidence for a cellular isoform of titin.
1992,
Pubmed
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Foltz,
Purification and characterization of an extracellular fragment of the sea urchin egg receptor for sperm.
1990,
Pubmed
,
Echinobase
Foltz,
Identification of the sea urchin egg receptor for sperm using an antiserum raised against a fragment of its extracellular domain.
1992,
Pubmed
,
Echinobase
Foltz,
Sea urchin egg receptor for sperm: sequence similarity of binding domain and hsp70.
1993,
Pubmed
,
Echinobase
Foltz,
The molecular basis of sea urchin gamete interactions at the egg plasma membrane.
1993,
Pubmed
,
Echinobase
Galione,
Redundant mechanisms of calcium-induced calcium release underlying calcium waves during fertilization of sea urchin eggs.
1993,
Pubmed
,
Echinobase
Guharay,
Stretch-activated single ion channel currents in tissue-cultured embryonic chick skeletal muscle.
1984,
Pubmed
Iwasa,
High concentration of inositol 1,4,5-trisphosphate in sea urchin sperm.
1990,
Pubmed
,
Echinobase
Jaffe,
Fast block to polyspermy in sea urchin eggs is electrically mediated.
1976,
Pubmed
,
Echinobase
Kinsey,
Purification and properties of the egg plasma membrane.
1986,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Larabell,
Fertilization-induced changes in the vitelline envelope of echinoderm and amphibian eggs: self-assembly of an extracellular matrix.
1991,
Pubmed
,
Echinobase
Lee,
Calcium mobilization by dual receptors during fertilization of sea urchin eggs.
1993,
Pubmed
,
Echinobase
Lomant,
Chemical probes of extended biological structures: synthesis and properties of the cleavable protein cross-linking reagent [35S]dithiobis(succinimidyl propionate).
1976,
Pubmed
Miyazaki,
Essential role of the inositol 1,4,5-trisphosphate receptor/Ca2+ release channel in Ca2+ waves and Ca2+ oscillations at fertilization of mammalian eggs.
1993,
Pubmed
,
Echinobase
Myles,
Molecular mechanisms of sperm-egg membrane binding and fusion in mammals.
1993,
Pubmed
Ohlendieck,
The sea urchin egg receptor for sperm: isolation and characterization of the intact, biologically active receptor.
1993,
Pubmed
,
Echinobase
Rossignol,
Characterization of the sperm receptor on the surface of eggs of Strongylocentrotus purpuratus.
1984,
Pubmed
,
Echinobase
Ruiz-Bravo,
Identification and partial characterization of sperm receptor associated with the newly formed fertilization envelope from sea urchin eggs.
1986,
Pubmed
,
Echinobase
Ruiz-Bravo,
Isolation and characterization of proteolytic fragments of the sea urchin sperm receptor that retain species specificity.
1986,
Pubmed
,
Echinobase
Schmell,
Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.
1977,
Pubmed
,
Echinobase
Swanson,
High affinity insulin binding in the human placenta insulin receptor requires alpha beta heterodimeric subunit interactions.
1989,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Treadway,
The endogenous functional turkey erythrocyte and rat liver insulin receptor is an alpha 2 beta 2 heterotetrameric complex.
1990,
Pubmed
Ullrich,
Signal transduction by receptors with tyrosine kinase activity.
1990,
Pubmed
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
