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Elife 2018 Jan 19;7. doi: 10.7554/eLife.31469.
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A disassembly-driven mechanism explains F-actin-mediated chromosome transport in starfish oocytes.

Bun P , Dmitrieff S , Belmonte JM , Nédélec FJ , Lénárt P .

While contraction of sarcomeric actomyosin assemblies is well understood, this is not the case for disordered networks of actin filaments (F-actin) driving diverse essential processes in animal cells. For example, at the onset of meiosis in starfish oocytes a contractile F-actin network forms in the nuclear region transporting embedded chromosomes to the assembling microtubule spindle. Here, we addressed the mechanism driving contraction of this 3D disordered F-actin network by comparing quantitative observations to computational models. We analyzed 3D chromosome trajectories and imaged filament dynamics to monitor network behavior under various physical and chemical perturbations. We found no evidence of myosin activity driving network contractility. Instead, our observations are well explained by models based on a disassembly-driven contractile mechanism. We reconstitute this disassembly-based contractile system in silico revealing a simple architecture that robustly drives chromosome transport to prevent aneuploidy in the large oocyte, a prerequisite for normal embryonic development.

PubMed ID: 29350616
PMC ID: PMC5788506
Article link: Elife
Grant support: [+]

Genes referenced: cep152 irak1bp1 LOC100893907 LOC115925415 LOC578403 LOC583082 LOC590297 pole

Article Images: [+] show captions
References [+] :
Almonacid, Active diffusion positions the nucleus in mouse oocytes. 2015, Pubmed