ECB-IMG-208616
Echinobase Image ID: 208616
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FIGURE 7. pFAK coats the spicule and overlaps with the edges of the microtubule filaments at 24hpf and 48hpf. pFAK. (A–H) Representative images showing sea urchin embryos stained with pFAK antibody (red), β‐Tubulin (green), and the skeletogenic cell marker 6a9 (blue) at 24hpf. (A–D) Mineralized grain area, where the mineral membrane is coated with active FAK (arrowhead) surrounded by thin microtubule layer. Arrows point to skeletogenic cells where microtubule filaments elongate from around the nucleus to the cell cortex. (E–H) The triradiate spicule area at 24hpf. The spicule membrane is coated with pFAK and the edges of the microtubule overlap with pFAK stain (arrowheads). Arrows point to skeletogenic cell where microtubule filaments elongate toward the spicule. The experiments were done in two biological replicates where the total number of embryos scored is 36. Scale bar in (A, D, E) is 10 μm. (I–P) Representative images showing a sea urchin larva stained with pFAK antibody (red) and β‐Tubulin (green) at 48hpf. (I, M) Whole embryo. (J–L) Zoom‐in into the body‐tip rectangle marked in (I). (N–P) Zoom‐in into the post oral‐tip rectangle marked in (M). White arrowheads point to the mineralized spicule where pFAK signal is high. White arrows point to skeletogenic cells extending microtubule filaments from around the nucleus into the spicule. Yellow arrowheads point where the elongated microtubule filaments overlap with pFAK. The experiments were done in two biological replicates where the total number of embryos scored is 17. Scale bar in (I, M) is 50 μm and in (L, P) is 10 μm. Image published in: Qassem A et al. (2025) Image downloaded from an Open Access article in PubMed Central. © 2025 The Author(s). Larger Image Printer Friendly View |