Echinobase Image ID: 208614

FIGURE 5. SM30 expression overlaps with the microtubule filaments in the skeletogenic lineage at 48hpf. Representative images showing sea urchin embryos stained with SM30 antibody (red), β‐Tubulin (green), and the skeletogenic cell marker 6a9 (blue) at 48hpf. (A–D) Whole embryo images. (E–H) Zoom‐in into body‐tip area marked by a rectangle in (A). (I–L) Zoom‐in into the body‐back area marked in (A), from a different embryo. (M–P) Zoom‐in into the tip of the post‐oral rod marked by a rectangle in (A). (Q–T) Zoom‐in into the anterolateral rod area marked by a rectangle in (A). Arrows point to skeletogenic cells where SM30 is enriched at the Golgi apparatus. White arrowheads point to the mineralized spicule where SM30 signal is high. Yellow arrowheads point to regions where SM30 signal is detected along the microtubule filaments that elongate from the Golgi apparatus toward the spicule. The experiments were done in three biological replicates where the total number of embryos scored is 30. Scale bar in (D) is 50 μm and (E, I, M, Q) is 10 μm. Image published in: Qassem A et al. (2025) Image downloaded from an Open Access article in PubMed Central. © 2025 The Author(s). Larger Image Printer Friendly View

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