Echinobase Image ID: 208591

Figure 4. PLIN2 interacts with ERP44 and TRXR2. (A,B) Predicted interactions of PLIN2 with ERP44 and TRXR2 based on AlphaFold3 modeling, visualized using PyMOL2. (C,D) Co-immunoprecipitation validation in HEK293T cells. Cells cultured in 10 cm dishes were co-transfected with PLIN2-EGFP and ERP44-HA or TRXR2-HA plasmids for 48 h. IP was implemented with anti-EGFP or anti-HA beads. IB was conducted with anti-HA and anti-EGFP antibodies, respectively; mouse IgG was used as the control. Data represent three independent biological replicates. (E,F) In vitro pull-down validation. Recombinant GST-PLIN2, His-ERP44, and TRXR2 were expressed in E. coli. His pull-down was performed by incubating His-ERP44 or His-TRXR2 with GST-PLIN2, followed by capture with His-affinity resin. Reciprocal GST pull-down assays were performed using GST-Sepharose resin. Bound proteins were analyzed by SDS-PAGE. The red arrow indicates the protein band pulled down after the interaction. ERP44, endoplasmic reticulum resident protein 44; PLIN2, perilipin 2; TRXR2, thioredoxin reductase 2, mitochondrial. Image published in: Fan H et al. (2026) Image downloaded from an Open Access article in PubMed Central. © 2026 by the authors. Larger Image Printer Friendly View

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