Echinobase Image ID: 197415

Figure 4. Actin polymerization and myosin activation affect skeletal growth and branching.(A–F) representative embryos showing the effect of actomyosin perturbations at 2df. (A) Control embryo (B) embryo treated with 2 μM Blebbistatin >25 hpf, (C) embryo treated with 2 μM Blebbistatin >20 hpf, (D) embryo treated with 2 nM Latrunculin-A and 1.5 μM Blebbistatin >25 hpf, (E) embryo treated with 2 nM Latrunculin-A >20 hpf, (F) embryo treated with 2 nM Latrunculin-A >25 hpf, arrow pointing to the additional spicule rod. (G) Statistics of Latrunculin-A and Blebbistatin phenotypes, color code of phenotype is indicated in the representative images. Error bars indicate standard deviation. All treatments were conducted in at least three biological replicates and the exact number of replicates and scored embryos are provided in Supplementary file 1d. (H–J) Representative spicules recorded at 72hpf from (H) control skeletogenic cell culture, (I) skeletogenic cell culture were 2 nM Latrunculin-A was added at 48hpf and (J) skeletogenic cell culture were 2 μM Blebbistatin was added at 48hpf. (K) Quantification of spicule length in the different treatments at 72hpf **p<0.001, Kruskal-Wallis non-parametric test. Results are based on three biological repeats for each treatment. Scale bars are 50 μm. Figure 4—source data 1. Phenotypes of Blebbistatin and Latrunculin-A (Lat-A) experiments in whole embryos presented in Figure 4G.Figure 4—source data 2. Measurements of spicule length in skeletogenic cell cultures in control, of Blebbistatin and Latrunculin-A (Lat-A), presented in Figure 4K. Image published in: Hijaze E et al. (2024) Image downloaded from an Open Access article in PubMed Central. © 2023, Hijaze et al Larger Image Printer Friendly View

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