Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1981 Aug 01;18:711-20. doi: 10.1128/mcb.1.8.711-720.1981.
Show Gene links
Show Anatomy links
Actin gene expression in developing sea urchin embryos.
Crain WR, Durica DS, Van Doren K.
???displayArticle.abstract???
We show that the synthesis of actin is regulated developmentally during early sea urchin embryogenesis and that the level of synthesis of this protein parallels the steady-state amounts of the actin messenger ribonucleic acids (RNA). An in vitro translation and RNA blotting analysis of embryo RNA from several stages of early development indicated that during the first 8 h after fertilization there was a low and relatively constant level of actin messenger RNA in the embryo. Between 8 and 13 h of development, the amount of actin messenger RNA began to increase both in the cytoplasm and on polysomes, and by 18 h the amounts of actin message per embryo had risen between approximately 10- and 25-fold in the cytoplasm and between 15- and 40-fold on polysomes. Two size classes of actin messenger RNA (2.2 and 1.8 kilobases) were identified in unfertilized eggs and in all of the developmental stages examined. The amount of each actin message class increased over a similar time interval during early development. However, the amounts of these size classes in the cytoplasm relative to each other shifted between the earliest stages examined (2 to 5 h) and the hatching blastula stage (18 h), with the ratio of the 1.8-kilobase actin messenger RNA to the 2.2-kilobase actin messenger RNA increasing almost threefold during this period.
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977, Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Berger,
Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
1979,
Pubmed Brandhorst,
Stabilities of nuclear and messenger RNA molecules in sea urchin embryos.
1972,
Pubmed
,
Echinobase Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Durica,
Organization of actin gene sequences in the sea urchin: molecular cloning of an intron-containing DNA sequence coding for a cytoplasmic actin.
1980,
Pubmed
,
Echinobase Gross,
The control of protein synthesis in embryonic development and differentiation.
1967,
Pubmed
,
Echinobase Hough-Evans,
Appearance and persistence of maternal RNA sequences in sea urchin development.
1977,
Pubmed
,
Echinobase Humphreys,
Measurements of messenger RNA entering polysomes upon fertilization of sea urchin eggs.
1971,
Pubmed
,
Echinobase Infante,
Accumulation of newly synthesized RNA templates in a unique class of polyribosomes during embryogenesis.
1967,
Pubmed Kedes,
Synthesis and function of messenger RNA during early embryonic development.
1969,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed Lasky,
Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAs.
1980,
Pubmed
,
Echinobase Lev,
Developmental expression of two cloned sequences coding for rare sea urchin embryo messages.
1980,
Pubmed
,
Echinobase Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed Merlino,
Cloning of sea urchin actin gene sequences for use in studying the regulation of actin gene transcription.
1980,
Pubmed
,
Echinobase Nemer,
Properties of sea urchin embryo messenger RNA containing and lacking poly(A).
1975,
Pubmed
,
Echinobase O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed Penman,
Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells.
1968,
Pubmed Pollard,
Actin and myosin and cell movement.
1974,
Pubmed Shepherd,
Developmental shifts in frequency distribution of polysomal mRNA and their posttranscriptional regulation in the sea urchin embryo.
1980,
Pubmed
,
Echinobase
