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Larger Image FIG. 4. XGREUL1 is expressed in the cement gland, cranial placodes, and the pronephros. Whole-mount in situ hybridization of Xenopus embryos using XGREUL1 antisense and sense probes. (A) Eight-cell-stage embryos; upper embryo showing GREUL1 expression in the animal hemisphere, the lower embryo is a sense control. (B) Anterior view of stage 20 embryos exhibiting cement gland staining, lower embryo is the sense control. Embryos were cleared with benzyl benzoate:benzyl alcohol (1:1). Stage 27 (C), stage 39 (D), and stage 42 (E) embryos expressing XGREUL1 in the lateral line system, the pronephros, the olfactory placode, and the otic vesicle. (F) RT-PCR showing GREUL1 expression during Xenopus development and using ODC as a control. Abbreviations: cg, cement gland; m, migratory primordium of middle trunk line; pAD, anterodorsal lateral line placode; pd, pronephric duct; g, pronephric tubules; pOl, olfactory placode; ot, otic vesicle. Image published in: Borchers AG et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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