FIG. 2. Expression of SGLT-1L is confined to tubules of the pronephric kidney. SGLT-1L and CLC-K transcripts were detected by whole-mount in situ hybridization. Lateral views of Xenopus embryos with anterior to the left are shown in (A-E, G, H). Transverse sections (F, I) are oriented with dorsal to the left. (A-D) Embryonic SGLT-1L expression started at stage 30 (A) and remained exclusively associated with the developing pronephric tubules throughout tadpole stages (B-D). (E) Enlargement of the pronephric region of the embryo shown in (D) illustrating SGLT-1L expression in connecting tubules (arrowheads) and the common tubule (arrow). (F) Transverse section of stage 37/38 embryo hybridized in whole mount for SGLT-1L expression. Embryos were embedded in plastic and 3 μm sections were cut at the level of the pronephros. SGLT- 1L transcripts were detected in tubular epithelia (pt), but not in the duct (pd). (G) Double in situ hybridization of a stage 36 embryo demonstrating SGLT-1L (magenta) and CLC-K (blue) expression. (H) A closeup view of the pronephric region of the embryo shown in (G). SGLT-1L expression is present in pronephric tubules (pt), whereas CLC-K transcripts are found in the pronephric duct (pd). Arrowheads indicate the junction between the common tubule and the duct. Selected epidermal cells express CLC-K (arrow). (I) Transverse section through the pronephric kidney of a stage 36 embryo hybridized in whole mount for SGLT-1L and CLC-K expression. The plane of section is indicated by the dotted line in (H). SGLT-1L and CLC-K transcripts were associated with distinct tubular (pt) and ductal (pd) epithelia, respectively.
Image published in: Eid SR et al. (2002)
Copyright © 2002. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| slc5a11.L | SGLT-1L | X. laevis | Throughout NF stage 29 and 30 to NF stage 33 and 34 | pronephric mesenchyme pronephric kidney |
| clcnkb.L | ClC-k, clc-kb, clckb, clcnka, clk-k2, LOC108703568, xClC-k | X. laevis | Throughout NF stage 35 and 36 | pronephric kidney pronephric tubule pronephric duct early distal tubule ciliated epidermal cell |
| slc5a11.L | SGLT-1L | X. laevis | Throughout NF stage 35 and 36 to NF stage 39 | kidney pronephric kidney early proximal tubule pronephric nephrostome pronephric nephron glomus glomeral nexus |
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