Fig. 3. Temporal and spatial expression of elrD transcripts. (A) One ïg of total RNA was reverse-transcribed and PCR performed by incorpo- rating digoxigenin-dUTP. PCR products were resolved by electrophore- sis in a 1% agarose gel and detected as described in Materials and Methods. Sequence analysis of the cloned products has shown that bands 1 and 2 correspond to elrD1 and elrD2 transcripts, respectively. Band 3 corresponds to an incomplete elongation of elrD1 and elrD2 transcripts. N: Neurula, T: Tailbud, B: Adult Brain. (B,C) In situ hybridiza- tion at tailbud stage with an antisense elrD1 (B) and elrD2 (C) probes. expression is seen in the brain vesicles (arrowheads), the cranial ganglia (arrows) and the spinal cord (block arrow).
Image published in: Nassar F (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| elavl4.L | elrD, elrD1, elrD2, HuD, LOC108715589 | X. laevis | Throughout NF stage 32 | nervous system brain spinal cord eye forebrain telencephalon midbrain hindbrain cephalic nerve olfactory bulb trigeminal nerve facial nerve glossopharyngeal nerve vagus nerve |
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