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Larger Image Fig. 2. Spatial pattern of hsp30 mRNA accumulation during early Xenopus development. Whole-mount in situ hybridization with DIGlabeled hsp30 antisense riboprobe was carried out with control (A,C,E) and heat shocked (B,D,F; 1 h at 33°C) Xenopus embryos at neurula (A,B; stage 17/18), mid- (C,D; stage 28) and late (E,F; stage 35) tailbud stages. At the late tailbud stage, control embryos (E) display a natural increase in eye pigmentation and melanocyte production. G,H: Control and heat shocked midtailbud stage embryos (stage 28), respectively, hybridized with DIG-labeled hsp30 sense riboprobe. I,J: Control and heat shocked midtailbud stage embryos (stage 28), respectively, hybridized with DIG-labeled actin antisense riboprobe. LP, lens placode; S, somites; P, proctodeum; CG, cement gland; SC, spinal cord; A, anus; ME, melanocytes. Image published in: Lang L et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher.
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