Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Asian Pac J Cancer Prev
2021 May 01;225:1365-1373. doi: 10.31557/APJCP.2021.22.5.1365.
Show Gene links
Show Anatomy links
Induction of Apoptosis by Acanthaster planci sp., and Diadema setosum sp., Fractions in Human Cervical Cancer Cell Line, HeLa.
Chaudhry GE
,
Islamiah M
,
Zafar MN
,
Bakar K
,
Aziz N
,
Saidin J
,
Sung YY
,
Tengku Muhammad TS
.
???displayArticle.abstract???
UNLABELLED: Cancer is an uncontrolled multiplication of cells. The desire efficacy and severe toxicity of current anticancer drugs urge exploring and investigating a better alternative to existing chemotherapeutics. Natural products of marine origin are excellent sources of potential new drugs of enhanced biological activities.
OBJECTIVES: Thus, the cytotoxic effects along with investigating the mode of cell death exerted by fractions, AP-9, AP-THR, DS-8 and DS-9 fraction of Acanthaster planci, Diadema setosum sp., on the human cervical cancer cell line, HeLa.
METHODS: The cytotoxicity of fractions has determined by using an MTS assay. The early and late apoptosis was studied by using the High content Screening (HCS) instrument.
RESULTS: The four fractions produced effective cytotoxicity effects with IC50 values at 72hr of less than 20 μg/ml in the order of AP-9 > DS-9 > APTHR-9 > DS-8. The fraction s exhibited cytotoxicity via mediating apoptotic mode of cell death. The early apoptosis by exposure of phosphatidylserine to the outer leaflet of the plasma membrane and late apoptosis due to the presence of green stain (DNA fragmentation) in treated cells.
CONCLUSION: The potent bioactive compounds might be responsible for inducing apoptosis in cancer cells and, thus, the potential to be a successful candidate for exploring upcoming chemotherapeutic drugs.
Figure 1B. Percentage of Growth Inhibition ± Standard Deviation for Acanthaster Planci (Thorn) APTHR-9 against HeLa Cells at 24 hr, 48 hr, and 72 hr
Figure 1C. Percentage of Growth Inhibition ± Standard Deviation for Diadema Setosum DS-8 against HeLa Cells at 24 hr, 48 hr, and 72 hr
Figure 1D. Percentage of Growth Inhibition ± Standard Deviation for Diadema Setosum DS-9 against HeLa Cells at 24hr, 48hr, and 72hr
Figure 2 (A-E). The Green Stain (Annexin-FITC) Early Apoptosis; Red stain, PI (propidium Iodide) late apoptosis indicates induction of apoptosis in HeLa cells; (A,B,C) AP-9 (D,E,F) DS-8 (G,H,I) DS-9 (J,K,L) APTHR (M,N,O) positive control (P,Q,R) Negative control (untreated) for 3hr, 6hr and 24hr respectively
Figure 3 (A-E). The Presence of Green Stain (FITC), which Indicates DNA Fragmentation in HeLa Cells Treated with Fractions Row A-E. (A) AP-9 (B) APTRN-9 (C) DS-8 (D) DS-9 (E) positive control (Vincristine sulfate) and (F) Negative control (without treatment), after 36 hr
Alison,
Apoptosis: a gene-directed programme of cell death.
1992, Pubmed
Alison,
Apoptosis: a gene-directed programme of cell death.
1992,
Pubmed
Alves,
The faunal drugstore: animal-based remedies used in traditional medicines in Latin America.
2011,
Pubmed
Alves,
Zootherapy goes to town: the use of animal-based remedies in urban areas of NE and N Brazil.
2007,
Pubmed
Amin,
Perspectives for cancer prevention with natural compounds.
2009,
Pubmed
Chaudhry,
Vitex rotundifolia fractions induce apoptosis in human breast cancer cell line, MCF-7, via extrinsic and intrinsic pathways.
2019,
Pubmed
Chaudhry,
Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway.
2020,
Pubmed
Chaudhry,
Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA.
2020,
Pubmed
Chaudhry,
Xylocarpus Moluccensis Induces Cytotoxicity in Human Hepatocellular Carcinoma HepG2 Cell Line via Activation of the Extrinsic Pathway.
2021,
Pubmed
Chaudhry,
Breast Cancer: A Global Concern, Diagnostic and Therapeutic Perspectives, Mechanistic Targets in Drug Development.
2021,
Pubmed
Cheng,
Asterosaponin 1, a cytostatic compound from the starfish Culcita novaeguineae, functions by inducing apoptosis in human glioblastoma U87MG cells.
2006,
Pubmed
,
Echinobase
Cheng,
Anti-tumor and pro-apoptotic activity of ethanolic extract and its various fractions from Polytrichum commune L.ex Hedw in L1210 cells.
2012,
Pubmed
Cotter,
Cell death via apoptosis and its relationship to growth, development and differentiation of both tumour and normal cells.
1990,
Pubmed
Cragg,
Natural products: a continuing source of novel drug leads.
2013,
Pubmed
Dong,
Chemical constituents and bioactivities of starfish.
2011,
Pubmed
,
Echinobase
Fadok,
Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages.
1992,
Pubmed
Inagaki,
Isolation and structure of four new ceramides from the starfish Luidia maculata.
2006,
Pubmed
,
Echinobase
Ishii,
A ceramide and cerebroside from the starfish asterias amurensis Lütken and their plant-growth promotion activities.
2006,
Pubmed
,
Echinobase
Jan,
Understanding Apoptosis and Apoptotic Pathways Targeted Cancer Therapeutics.
2019,
Pubmed
Ke,
The anti-lung cancer activity of SEP is mediated by the activation and cytotoxicity of NK cells via TLR2/4 in vivo.
2014,
Pubmed
,
Echinobase
Lee,
Activation of p53 with ilimaquinone and ethylsmenoquinone, marine sponge metabolites, induces apoptosis and autophagy in colon cancer cells.
2015,
Pubmed
Murray,
Sustainable production of biologically active molecules of marine based origin.
2013,
Pubmed
Sahara,
In vivo anti-tumour effect of 3'-sulphonoquinovosyl 1'-monoacylglyceride isolated from sea urchin (Strongylocentrotus intermedius) intestine.
1997,
Pubmed
,
Echinobase
Sawadogo,
A Survey of Marine Natural Compounds and Their Derivatives with Anti-cancer Activity Reported in 2012.
2015,
Pubmed
Schumacher,
Gold from the sea: marine compounds as inhibitors of the hallmarks of cancer.
2011,
Pubmed
Tang,
Asterosaponins from the starfish Culcita novaeguineae and their bioactivities.
2006,
Pubmed
,
Echinobase
Thao,
Asterosaponins from the Starfish Astropecten monacanthus suppress growth and induce apoptosis in HL-60, PC-3, and SNU-C5 human cancer cell lines.
2014,
Pubmed
,
Echinobase
Venter,
Environmental genome shotgun sequencing of the Sargasso Sea.
2004,
Pubmed
Williams,
Why are secondary metabolites (natural products) biosynthesized?
1989,
Pubmed
Wu,
Heteronemin, a Spongean Sesterterpene, Induces Cell Apoptosis and Autophagy in Human Renal Carcinoma Cells.
2015,
Pubmed
Zafar,
Synthesis, characterization and anti-cancer properties of water-soluble bis(PYE) pro-ligands and derived palladium(ii) complexes.
2019,
Pubmed
Zhang,
Cytotoxic triterpene glycosides from the sea cucumber Pseudocolochirus violaceus.
2007,
Pubmed
,
Echinobase
Zhao,
Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells.
2011,
Pubmed
,
Echinobase
Zhou,
Novaeguinoside II inhibits cell proliferation and induces apoptosis of human brain glioblastoma U87MG cells through the mitochondrial pathway.
2011,
Pubmed
,
Echinobase
