ECB-ART-43805
Dev Biol
2015 Jun 01;4021:127-41. doi: 10.1016/j.ydbio.2015.01.008.
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microRNAs regulate β-catenin of the Wnt signaling pathway in early sea urchin development.
Stepicheva N
,
Nigam PA
,
Siddam AD
,
Peng CF
,
Song JL
.
Abstract
Development of complex multicellular organisms requires careful regulation at both transcriptional and post-transcriptional levels. Post-transcriptional gene regulation is in part mediated by a class of non-coding RNAs of 21-25 nucleotides in length known as microRNAs (miRNAs). β-catenin, regulated by the canonical Wnt signaling pathway, has a highly evolutionarily conserved function in patterning early metazoan embryos, in forming the Anterior-Posterior axis, and in establishing the endomesoderm. Using reporter constructs and site-directed mutagenesis, we identified at least three miRNA binding sites within the 3'' untranslated region (3''UTR) of the sea urchin β-catenin. Further, blocking these three miRNA binding sites within the β-catenin 3''UTR to prevent regulation of endogenous β-catenin by miRNAs resulted in a minor increase in β-catenin protein accumulation that is sufficient to induce aberrant gut morphology and circumesophageal musculature. These phenotypes are likely the result of increased transcript levels of Wnt responsive endomesodermal regulatory genes. This study demonstrates the importance of miRNA regulation of β-catenin in early development.
PubMed ID: 25614238
PMC ID: PMC4433612
Article link: Dev Biol
Grant support: [+]
P20 GM103446 NIGMS NIH HHS , P20 GM103653 NIGMS NIH HHS , NIHNIGMS5P20GM103653-02 PHS HHS , NIHNIGMS8P20GM103446-12 PHS HHS
Genes referenced: LOC100887844 LOC594353
Antibodies: LOC580674 Ab1 ctnnb1 Ab2 ctnnb1 Ab3 msp130 Ab2
Morpholinos: ctnnb1 MO1 ctnnb1 MO2 dicer1 MO1
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