Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Biol 2009 Nov 15;3352:428-41. doi: 10.1016/j.ydbio.2009.08.005.
Show Gene links Show Anatomy links

The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo.

Wahl ME , Hahn J , Gora K , Davidson EH , Oliveri P .

The genomic cis-regulatory systems controlling regulatory gene expression usually include multiple modules. The regulatory output of such systems at any given time depends on which module is directing the function of the basal transcription apparatus, and ultimately on the transcription factor inputs into that module. Here we examine regulation of the Strongylocentrotus purpuratus tbrain gene, a required activator of the skeletogenic specification state in the lineage descendant from the embryo micromeres. Alternate cis-regulatory modules were found to convey skeletogenic expression in reporter constructs. To determine their relative developmental functions in context, we made use of recombineered BAC constructs containing a GFP reporter and of derivatives from which specific modules had been deleted. The outputs of the various constructs were observed spatially by GFP fluorescence and quantitatively over time by QPCR. In the context of the complete genomic locus, early skeletogenic expression is controlled by an intron enhancer plus a proximal region containing a HesC site as predicted from network analysis. From ingression onward, however, a dedicated distal module utilizing positive Ets1/2 inputs contributes to definitive expression in the skeletogenic mesenchyme. This module also mediates a newly discovered negative Erg input which excludes non-skeletogenic mesodermal expression.

PubMed ID: 19679118
PMC ID: PMC2784196
Article link: Dev Biol
Grant support: [+]

Genes referenced: erg ets1 LOC100887844 LOC592057
Morpholinos: elk1 MO1 ets1 MO1 etv6 MO1 fgfr2l MO1 spdef MO1

References [+] :
Cameron, cis-Regulatory activity of randomly chosen genomic fragments from the sea urchin. 2004, Pubmed, Echinobase