Figure 5. Hp-Htt (red)/BrdU (green)/nuclei (blue) triple stained image of 4aPL. Shortened larval arms due to FL-Htt-MO application occurred, accompanied by decreased BrdU-positive signals, particularly at the larval arm regions. (A) Dorsal view of the larva. Hp-Htt was detected at the CBAS (large arrow) and some of the blastocoelar cells (small arrows). (B) The highly magnified CBAS area shown by a rectangle (b) in (A). BrdU was detected also there (arrows). (C) Hp-Htt/DAPI double stained image of (A). Arrow; CBAS. Hp-Htt-positive blastocoelar cell network (asterisk). (D) At the ectoderm, a smaller number of BrdU-positive signals were detected in the blastocoelar cells (arrows) (E) Ventral view of the FL-Htt-MO treated larva. The number of BrdU-positive signals clearly decreased in the ectoderm, particularly at the tip of arms and in the fragmented CBAS (double-head arrow). (F) The highly magnified area shown by a rectangle (f) in (E). Shorter arm length is clear at the postoral arms (POA). (G) Fragmented CBAS (double-head arrow in F and G). The blastocoelar Hp-Htt-positive cell network (asterisk) is rather unaffected. (H) BrdU-positive signal image extracted from (E) clearly detecting the declined positive signal at the tip regions of the arms (arrows), while those in the blastocoel were not apparently decreased (small arrows). (I) Statistical analysis of average arm length comparison between control larvae and FL-Htt-MO-applied larvae with p-value. The larval number examined are shown in a box on each column. Scale bars = 50 µm (A,C,E,G). 25 µm (B,F).
Image published in: Katow H et al. (2021)
Image downloaded from an Open Access article in PubMed Central. © 2021 by the authors.
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