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Abstract
We previously demonstrated that the flagellin of intracellular Vibrio splendidus AJ01 could be specifically identified by tropomodulin (Tmod) and further mediate p53-dependent coelomocyte apoptosis in the sea cucumber Apostichopus japonicus. In higher animals, Tmod serves as a regulator in stabilizing the actin cytoskeleton. However, the mechanism on how AJ01 breaks the AjTmod-stabilized cytoskeleton for internalization remains unclear. Here, we identified a novel AJ01 Type III secretion system (T3SS) effector of leucine-rich repeat-containing serine/threonine-protein kinase (STPKLRR) with five LRR domains and a serine/threonine kinase (STYKc) domain, which could specifically interact with tropomodulin domain of AjTmod. Furthermore, we found that STPKLRR directly phosphorylated AjTmod at serine 52 (S52) to reduce the binding stability between AjTmod and actin. After AjTmod dissociated from actin, the F-actin/G-actin ratio decreased to induce cytoskeletal rearrangement, which in turn promoted the internalization of AJ01. The STPKLRR knocked out strain could not phosphorylated AjTmod and displayed lower internalization capacity and pathogenic effect compared to AJ01. Overall, we demonstrated for the first time that the T3SS effector STPKLRR with kinase activity was a novel virulence factor in Vibrio and mediated self-internalization by targeting host AjTmod phosphorylation dependent cytoskeleton rearrangement, which provided a candidate target to control AJ01 infection in practice.
Aktories,
Actin as target for modification by bacterial protein toxins.
2011, Pubmed
Aktories,
Actin as target for modification by bacterial protein toxins.
2011,
Pubmed
Alber,
Signaling mechanisms of the Mycobacterium tuberculosis receptor Ser/Thr protein kinases.
2009,
Pubmed
Alberti-Segui,
Differential function of Listeria monocytogenes listeriolysin O and phospholipases C in vacuolar dissolution following cell-to-cell spread.
2007,
Pubmed
Barford,
Molecular mechanisms of the protein serine/threonine phosphatases.
1996,
Pubmed
Barger,
F-actin organization and target constriction during primary macrophage phagocytosis is balanced by competing activity of myosin-I and myosin-II.
2022,
Pubmed
Bennett,
Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues.
2001,
Pubmed
Bogoyevitch,
Peptide inhibitors of protein kinases-discovery, characterisation and use.
2005,
Pubmed
Bonazzi,
Clathrin phosphorylation is required for actin recruitment at sites of bacterial adhesion and internalization.
2011,
Pubmed
Bonazzi,
Successive post-translational modifications of E-cadherin are required for InlA-mediated internalization of Listeria monocytogenes.
2008,
Pubmed
Bruno,
Salmonella Typhimurium type III secretion effectors stimulate innate immune responses in cultured epithelial cells.
2009,
Pubmed
Canova,
Bacterial serine/threonine protein kinases in host-pathogen interactions.
2014,
Pubmed
Chai,
New insights into the evasion of host innate immunity by Mycobacterium tuberculosis.
2020,
Pubmed
Chai,
A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin.
2022,
Pubmed
Chauhan,
Pathogenicity and Virulence of Legionella: Intracellular replication and host response.
2021,
Pubmed
Chen,
A unique NLRC4 receptor from echinoderms mediates Vibrio phagocytosis via rearrangement of the cytoskeleton and polymerization of F-actin.
2021,
Pubmed
,
Echinobase
Chen,
The Bacterial T6SS Effector EvpP Prevents NLRP3 Inflammasome Activation by Inhibiting the Ca2+-Dependent MAPK-Jnk Pathway.
2017,
Pubmed
Cheng,
Molecular mechanisms of cardiovascular toxicity of targeted cancer therapeutics.
2010,
Pubmed
Christie,
Biogenesis, architecture, and function of bacterial type IV secretion systems.
2005,
Pubmed
Dai,
Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata.
2022,
Pubmed
,
Echinobase
Dai,
FliC of Vibrio splendidus-related strain involved in adhesion to Apostichopus japonicus.
2020,
Pubmed
,
Echinobase
Foussard,
The molecular puzzle of two-component signaling cascades.
2001,
Pubmed
Fowler,
Regulation of actin filament length in erythrocytes and striated muscle.
1996,
Pubmed
Galán,
Protein delivery into eukaryotic cells by type III secretion machines.
2006,
Pubmed
Gerhard,
Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB.
2005,
Pubmed
Giacaman,
Sugars and beyond. The role of sugars and the other nutrients and their potential impact on caries.
2018,
Pubmed
Greenstein,
Structure/function studies of Ser/Thr and Tyr protein phosphorylation in Mycobacterium tuberculosis.
2005,
Pubmed
Gregorio,
Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly.
1995,
Pubmed
Hanks,
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
1995,
Pubmed
Hochmann,
Salmonella enterica SpvB ADP-ribosylates actin at position arginine-177-characterization of the catalytic domain within the SpvB protein and a comparison to binary clostridial actin-ADP-ribosylating toxins.
2006,
Pubmed
Hosokawa,
Quantitative measurement of in vivo phosphorylation states of Cdk5 activator p35 by Phos-tag SDS-PAGE.
2010,
Pubmed
Hotinger,
Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.
2021,
Pubmed
Huang,
mTORC2 controls actin polymerization required for consolidation of long-term memory.
2013,
Pubmed
Hume,
Phosphorylation of retinoblastoma protein by viral protein with cyclin-dependent kinase function.
2008,
Pubmed
Håkansson,
The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane.
1996,
Pubmed
Jin,
Identification and biochemical characterization of a eukaryotic-type serine/threonine kinase and its cognate phosphatase in Streptococcus pyogenes: their biological functions and substrate identification.
2006,
Pubmed
Johnson,
Pathogenicity of Toxoplasma gondii cysts and oocysts measured by fever and weight loss in mice.
1988,
Pubmed
Knighton,
Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.
1991,
Pubmed
Kostyukova,
Tropomodulins and tropomodulin/tropomyosin interactions.
2008,
Pubmed
Kumari,
Tropomodulins Control the Balance between Protrusive and Contractile Structures by Stabilizing Actin-Tropomyosin Filaments.
2020,
Pubmed
Lewis,
Invasion of Host Cells and Tissues by Uropathogenic Bacteria.
2016,
Pubmed
Li,
A manganese superoxide dismutase in blood clam Tegillarca granosa: molecular cloning, tissue distribution and expression analysis.
2011,
Pubmed
Liang,
Vibrio splendidus virulence to Apostichopus japonicus is mediated by hppD through glutamate metabolism and flagellum assembly.
2022,
Pubmed
,
Echinobase
Lim,
Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling.
2015,
Pubmed
Lima,
Serine/threonine protein kinase PrkA of the human pathogen Listeria monocytogenes: biochemical characterization and identification of interacting partners through proteomic approaches.
2011,
Pubmed
Liu,
Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka).
2010,
Pubmed
,
Echinobase
Lv,
miR-137 modulates coelomocyte apoptosis by targeting 14-3-3ζ in the sea cucumber Apostichopus japonicus.
2017,
Pubmed
,
Echinobase
Miao,
An infection-induced RhoB-Beclin 1-Hsp90 complex enhances clearance of uropathogenic Escherichia coli.
2021,
Pubmed
Miller,
A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.
2017,
Pubmed
Muñoz-Dorado,
A gene encoding a protein serine/threonine kinase is required for normal development of M. xanthus, a gram-negative bacterium.
1991,
Pubmed
Mylvaganam,
The cytoskeleton in phagocytosis and macropinocytosis.
2021,
Pubmed
Ortiz-Lombardía,
Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis.
2003,
Pubmed
Park,
Outer membrane vesicles as a candidate vaccine against edwardsiellosis.
2011,
Pubmed
Pradhan,
Mycobacterial PknG Targets the Rab7l1 Signaling Pathway To Inhibit Phagosome-Lysosome Fusion.
2018,
Pubmed
Sachsenmaier,
Targeting protein kinases for tumor therapy.
2001,
Pubmed
Sandkvist,
General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio cholerae.
1997,
Pubmed
Schafer,
Control of actin assembly at filament ends.
1995,
Pubmed
Schwechheimer,
Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions.
2015,
Pubmed
Shao,
Xenophagy of invasive bacteria is differentially activated and modulated via a TLR-TRAF6-Beclin1 axis in echinoderms.
2022,
Pubmed
,
Echinobase
Sherr,
Use of monodispersed, fluorescently labeled bacteria to estimate in situ protozoan bacterivory.
1987,
Pubmed
Shimizu,
Cloning and chromosome assignment to 1q32 of a human cDNA (RAB7L1) encoding a small GTP-binding protein, a member of the RAS superfamily.
1997,
Pubmed
Sousa,
Src, cortactin and Arp2/3 complex are required for E-cadherin-mediated internalization of Listeria into cells.
2007,
Pubmed
Tsurumura,
Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex.
2013,
Pubmed
Vanhove,
Outer membrane vesicles are vehicles for the delivery of Vibrio tasmaniensis virulence factors to oyster immune cells.
2015,
Pubmed
Visschedyk,
Photox, a novel actin-targeting mono-ADP-ribosyltransferase from Photorhabdus luminescens.
2010,
Pubmed
Wang,
A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice.
1998,
Pubmed
Wang,
Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy.
2018,
Pubmed
Weber,
Subversion of the Endocytic and Secretory Pathways by Bacterial Effector Proteins.
2018,
Pubmed
Weber,
Tropomodulin caps the pointed ends of actin filaments.
1994,
Pubmed
Welch,
Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes.
1997,
Pubmed
Wennerberg,
Rho-family GTPases: it's not only Rac and Rho (and I like it).
2004,
Pubmed
Woida,
The Vibrio cholerae MARTX toxin silences the inflammatory response to cytoskeletal damage before inducing actin cytoskeleton collapse.
2020,
Pubmed
Yamashiro,
Tropomodulins: pointed-end capping proteins that regulate actin filament architecture in diverse cell types.
2012,
Pubmed
Zhuang,
Cloning and characterization of the virulence factor Hop from Vibrio splendidus.
2020,
Pubmed
,
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