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Nucleic Acids Res 2012 Dec 01;4022:e173. doi: 10.1093/nar/gks765.
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Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation.

Wada T , Hara M , Taneda T , Qingfu C , Takata R , Moro K , Takeda K , Kishimoto T , Handa H .

Gene downregulation by antisense morpholino oligonucleotides (MOs) is achieved by either hybridization around the translation initiation codon or by targeting the splice donor site. In the present study, an antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstream from a poly(A) tail junction in the 3''-untranslated region (UTR) of maternal mRNA. The MO removed the poly(A) tail and blocked zebrafish cdk9 (zcdk9) mRNA translation, showing functional mimicry between miRNA and MO. A PCR-based assay revealed MO-mediated specific poly(A) tail removal of zebrafish mRNAs, including those for cyclin B1, cyclin B2 and tbp. The MO activity targeting cyclins A and B mRNAs was validated in unfertilized starfish oocytes and eggs. The MO removed the elongated poly(A) tail from maternal matured mRNA. This antisense method introduces a new application for the targeted downregulation of maternal mRNAs in animal oocytes, eggs and early embryos.

PubMed ID: 22904086
PMC ID: PMC3526265
Article link: Nucleic Acids Res

Species referenced: Echinodermata
Genes referenced: cdk9 LOC115919910 LOC583082 LOC590297 mos tbp
Morpholinos: ccna2 MO2 ccnb2 MO3 ccnb2 MO4 ccnb2 MO5 cdk9 MO1 cdk9 MO10 cdk9 MO2 cdk9 MO3 cdk9 MO4 cdk9 MO5 cdk9 MO6 cdk9 MO7 cdk9 MO8 cdk9 MO9 tbp MO1

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References [+] :
Bill, A primer for morpholino use in zebrafish. 2009, Pubmed