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MAP kinase links the fertilization signal transduction pathway to the G1/S-phase transition in starfish eggs.
Tachibana K
,
Machida T
,
Nomura Y
,
Kishimoto T
.
Abstract
The mechanism by which fertilization initiates S-phase in the zygote is examined by manipulating the activity of MAP kinase in mature starfish eggs. These unfertilized eggs, which are arrested at G1-phase after the completion of meiosis, have high MAP kinase activity but undetectable cdc2 kinase activity. Either fertilization or inhibition of protein synthesis causes a decrease in MAP kinase activity, which is followed by DNA synthesis. Inactivation of MAP kinase with its specific phosphatase, CL100, initiates DNA synthesis in the absence of fertilization, while constitutive activation of MAP kinase with MEK represses the initiation of DNA synthesis following fertilization. Thus, in unfertilized mature starfish eggs, a capacity for DNA replication is already acquired, but entry into S-phase is negatively regulated by MAP kinase activity that is supported by a continuously synthesized protein(s) but not by cdc2 kinase. Upon fertilization, downregulation of MAP kinase activity is necessary and sufficient for triggering the G1/S-phase transition.
Alessi,
The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts.
1993, Pubmed
Alessi,
The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts.
1993,
Pubmed
Axton,
The inhibitor of DNA replication encoded by the Drosophila gene plutonium is a small, ankyrin repeat protein.
1994,
Pubmed
Cano,
Parallel signal processing among mammalian MAPKs.
1995,
Pubmed
Choi,
Mos/mitogen-activated protein kinase can induce early meiotic phenotypes in the absence of maturation-promoting factor: a novel system for analyzing spindle formation during meiosis I.
1996,
Pubmed
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
Deshaies,
Make it or break it: the role of ubiquitin-dependent proteolysis in cellular regulation.
1995,
Pubmed
Edgar,
Developmental control of cell cycle regulators: a fly's perspective.
1996,
Pubmed
Freeman,
The gnu mutation of Drosophila causes inappropriate DNA synthesis in unfertilized and fertilized eggs.
1987,
Pubmed
Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
Herskowitz,
MAP kinase pathways in yeast: for mating and more.
1995,
Pubmed
Huang,
Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system.
1996,
Pubmed
Huang,
Constitutive activation of Mek1 by mutation of serine phosphorylation sites.
1994,
Pubmed
Kishimoto,
Starfish maturation-promoting factor.
1996,
Pubmed
,
Echinobase
Kishimoto,
Microinjection and cytoplasmic transfer in starfish oocytes.
1986,
Pubmed
,
Echinobase
Kosako,
Mitogen-activated protein kinase kinase is required for the mos-induced metaphase arrest.
1994,
Pubmed
Kosako,
Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.
1994,
Pubmed
Labib,
rum1: a CDK inhibitor regulating G1 progression in fission yeast.
1996,
Pubmed
McKinney,
Negative regulation of FAR1 at the Start of the yeast cell cycle.
1993,
Pubmed
Meijer,
Maturation and fertilization in starfish oocytes.
1984,
Pubmed
,
Echinobase
Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
Nigg,
Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle.
1995,
Pubmed
Nomura,
Initiation of DNA replication cycle in fertilized eggs of the starfish, Asterina pectinifera.
1991,
Pubmed
,
Echinobase
Nurse,
Ordering S phase and M phase in the cell cycle.
1994,
Pubmed
Ohsumi,
Meiosis-specific cell cycle regulation in maturing Xenopus oocytes.
1994,
Pubmed
Okumura,
Initial triggering of M-phase in starfish oocytes: a possible novel component of maturation-promoting factor besides cdc2 kinase.
1996,
Pubmed
,
Echinobase
Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed
,
Echinobase
Peter,
Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.
1994,
Pubmed
Peter,
FAR1 links the signal transduction pathway to the cell cycle machinery in yeast.
1993,
Pubmed
Picard,
Newly assembled cyclin B-cdc2 kinase is required to suppress DNA replication between meiosis I and meiosis II in starfish oocytes.
1996,
Pubmed
,
Echinobase
Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
Sagata,
Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance.
1996,
Pubmed
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
Shamanski,
The Drosophila plutonium and pan gu genes regulate entry into S phase at fertilization.
1991,
Pubmed
Sherr,
Inhibitors of mammalian G1 cyclin-dependent kinases.
1995,
Pubmed
Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
Shibuya,
Activation of p42 MAP kinase and the release of oocytes from cell cycle arrest.
1992,
Pubmed
Shimada,
Discontinuous DNA replication in developing sea urchin embryos.
1976,
Pubmed
,
Echinobase
Tyers,
Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes.
1993,
Pubmed
Zhang,
Differential replication capacities of G1 and S-phase extracts from sea urchin eggs.
1993,
Pubmed
,
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