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Mol Biol Cell 2003 Nov 01;1411:4685-94. doi: 10.1091/mbc.e03-04-0249.
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Localization and dynamics of Cdc2-cyclin B during meiotic reinitiation in starfish oocytes.

Terasaki M , Okumura E , Hinkle B , Kishimoto T .

The Cdc2-cyclin B kinase has a central role in regulating the onset of M phase. In starfish oocytes, Cdc2-cyclin B begins to be activated approximately 10 min after application of maturation hormone, followed by accumulation in the nucleus then nuclear envelope breakdown. By immunofluorescence and by expressing a green fluorescent (GFP) chimera of cyclin B, we find that cyclin B is present in aggregates in the cytoplasm of immature oocytes. The aggregates disperse at approximately 10 min, suggesting that the dispersal is closely related to the activation of the kinase. Using cyclin B-GFP, the dispersion begins from the region containing the centrosomes. Extractability of Cdc2-cyclin B changes with similar kinetics during maturation. Active Cdc25 phosphatase released Cdc2-cyclin B from the detergent-insoluble fraction independently of its phosphatase activity. Live cell imaging also showed that Cdc2-cyclin B begins to accumulate in the nucleus before changes in nuclear pore permeability, consistent with Cdc2-cyclin B-induced disassembly of the pores.

PubMed ID: 14551249
PMC ID: PMC266783
Article link: Mol Biol Cell
Grant support: [+]

Genes referenced: LOC115919910 LOC574811 LOC576121

References [+] :
Beckhelling, Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts. 2003, Pubmed