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ECB-ART-37398
Mol Biol Cell 2000 Mar 01;113:897-914. doi: 10.1091/mbc.11.3.897.
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Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.

Terasaki M .


Abstract
The endoplasmic reticulum (ER) and Golgi were labeled by green fluorescent protein chimeras and observed by time-lapse confocal microscopy during the rapid cell cycles of sea urchin embryos. The ER undergoes a cyclical microtubule-dependent accumulation at the mitotic poles and by photobleaching experiments remains continuous through the cell cycle. Finger-like indentations of the nuclear envelope near the mitotic poles appear 2-3 min before the permeability barrier of the nuclear envelope begins to change. This permeability change in turn is approximately 30 s before nuclear envelope breakdown. During interphase, there are many scattered, disconnected Golgi stacks throughout the cytoplasm, which appear as 1- to 2-microm fluorescent spots. The number of Golgi spots begins to decline soon after nuclear envelope breakdown, reaches a minimum soon after cytokinesis, and then rapidly increases. At higher magnification, smaller spots are seen, along with increased fluorescence in the ER. Quantitative measurements, along with nocodazole and photobleaching experiments, are consistent with a redistribution of some of the Golgi to the ER during mitosis. The scattered Golgi coalesce into a single large aggregate during the interphase after the ninth embryonic cleavage; this is likely to be preparatory for secretion of the hatching enzyme during the following cleavage cycle.

PubMed ID: 10712508
PMC ID: PMC14819
Article link: Mol Biol Cell


Genes referenced: LOC100887844 LOC115919910 LOC115925415 mmp7

References [+] :
Acharya, Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis. 1998, Pubmed