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Vibrio splendidus AJ01 Promotes Pathogenicity via L-Glutamic Acid.
Li Y
,
Shi W
,
Zhang W
.
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Vibrio splendidus is a pathogen that infects a wide range of hosts, especially the sea cucumber species Apostichopus japonicus. Previous studies showed that the level of L-glutamic acid (L-Glu) significantly increased under heat stress, and it was found to be one of the best carbon sources used by V. splendidus AJ01. In this study, the effects of exogenous L-Glu on the coelomocyte viability, tissue status, and individual mortality of sea cucumbers were analyzed. The results showed that 10 mM of L-Glu decreased coelomocyte viability and increased individual mortality, with tissue rupture and pyknosis, while 0.1 mM of L-Glu slightly affected the survival of sea cucumbers without obvious damage at the cellular and tissue levels. Transcriptomic analysis showed that exogenous L-Glu upregulated 343 and downregulated 206 genes. Gene Ontology (GO) analysis showed that differentially expressed genes (DEGs) were mainly enriched in signaling and membrane formation, while a Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in the upregulated endocytosis and downregulated lysosomal pathways. The coelomocyte viability further decreased by 20% in the simultaneous presence of exogenous L-Glu and V. splendidus AJ01 compared with that in the presence of V. splendidus AJ01 infection alone. Consequently, a higher sea cucumber mortality was also observed in the presence of exogenous L-Glu challenged by V. splendidus AJ01. Real-time reverse transcriptase PCR showed that L-Glu specifically upregulated the expression of the fliC gene coding the subunit protein of the flagellar filament, promoting the swimming motility activity of V. splendidus. Our results indicate that L-Glu should be kept in a state of equilibrium, and excess L-Glu at the host-pathogen interface prompts the virulence of V. splendidus via the increase of bacterial motility.
LR20C190001 Zhejiang Provincial Natural Science Foundation, 31972833 the National Natural Science Foundation of China, N/A the K.C. Wong Magna Fund at Ningbo University
Binesse,
Metalloprotease vsm is the major determinant of toxicity for extracellular products of Vibrio splendidus.
2008, Pubmed
Binesse,
Metalloprotease vsm is the major determinant of toxicity for extracellular products of Vibrio splendidus.
2008,
Pubmed
Brosnan,
Glutamate: a truly functional amino acid.
2013,
Pubmed
Buratta,
Lysosomal Exocytosis, Exosome Release and Secretory Autophagy: The Autophagic- and Endo-Lysosomal Systems Go Extracellular.
2020,
Pubmed
Charles,
Experimental infection of Mytilus edulis by two Vibrio splendidus-related strains: Determination of pathogenicity level of strains and influence of the origin and annual cycle of mussels on their sensitivity.
2020,
Pubmed
Chen,
A unique NLRC4 receptor from echinoderms mediates Vibrio phagocytosis via rearrangement of the cytoskeleton and polymerization of F-actin.
2021,
Pubmed
,
Echinobase
Cossart,
Endocytosis of viruses and bacteria.
2014,
Pubmed
D'Souza-Schorey,
ARF proteins: roles in membrane traffic and beyond.
2006,
Pubmed
Dai,
FliC of Vibrio splendidus-related strain involved in adhesion to Apostichopus japonicus.
2020,
Pubmed
,
Echinobase
Dai,
Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata.
2022,
Pubmed
,
Echinobase
Dai,
Novel secreted STPKLRR from Vibrio splendidus AJ01 promotes pathogen internalization via mediating tropomodulin phosphorylation dependent cytoskeleton rearrangement.
2023,
Pubmed
,
Echinobase
Duperthuy,
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas.
2010,
Pubmed
Gao,
Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge.
2015,
Pubmed
,
Echinobase
Garnier,
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas.
2007,
Pubmed
Gatesoupe,
Pathogenicity of vibrio splendidus strains associated with turbot larvae, scophthalmus maximus.
1999,
Pubmed
Gevers,
Opinion: Re-evaluating prokaryotic species.
2005,
Pubmed
Goto,
Exogenous Treatment with Glutamate Induces Immune Responses in Arabidopsis.
2020,
Pubmed
Jiang,
Vibrio Clade 3.0: New Vibrionaceae Evolutionary Units Using Genome-Based Approach.
2021,
Pubmed
Jiang,
The pan-genome of Splendidus clade species in the family Vibrionaceae: Insights into evolution, adaptation, and pathogenicity.
2022,
Pubmed
Jiang,
Selection of the Amino Acid and Saccharide That Increase the Tetracycline Susceptibility of Vibrio splendidus.
2021,
Pubmed
Kim,
HISAT: a fast spliced aligner with low memory requirements.
2015,
Pubmed
Li,
Nutrition and metabolism of glutamate and glutamine in fish.
2020,
Pubmed
Liu,
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment.
2013,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Love,
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.
2014,
Pubmed
Lv,
IL-17/IL-17 Receptor Pathway-Mediated Inflammatory Response in Apostichopus japonicus Supports the Conserved Functions of Cytokines in Invertebrates.
2022,
Pubmed
,
Echinobase
Nishiyama,
Mlp24 (McpX) of Vibrio cholerae implicated in pathogenicity functions as a chemoreceptor for multiple amino acids.
2012,
Pubmed
O'Malley,
Plant-exuded chemical signals induce surface attachment of the bacterial pathogen Pseudomonas syringae.
2023,
Pubmed
Olive,
Metabolic crosstalk between host and pathogen: sensing, adapting and competing.
2016,
Pubmed
Oyanedel,
Vibrio splendidus O-antigen structure: a trade-off between virulence to oysters and resistance to grazers.
2020,
Pubmed
Pashaei,
The potential roles of amino acids and their major derivatives in the management of multiple sclerosis.
2022,
Pubmed
Reiling,
A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.
2013,
Pubmed
Ren,
Amino Acids As Mediators of Metabolic Cross Talk between Host and Pathogen.
2018,
Pubmed
Scharf,
Chemotaxis signaling systems in model beneficial plant-bacteria associations.
2016,
Pubmed
Schousboe,
Glutamate metabolism in the brain focusing on astrocytes.
2014,
Pubmed
Shen,
Ralstonia solanacearum promotes pathogenicity by utilizing l-glutamic acid from host plants.
2020,
Pubmed
Tattoli,
Amino acid starvation induced by invasive bacterial pathogens triggers an innate host defense program.
2012,
Pubmed
Vanhove,
Outer membrane vesicles are vehicles for the delivery of Vibrio tasmaniensis virulence factors to oyster immune cells.
2015,
Pubmed
Yang,
Test for l-glutamate inhibition of growth of Alternaria alternata by inducing resistance in tomato fruit.
2017,
Pubmed
Zhang,
iTRAQ-based proteomics reveals novel members involved in pathogen challenge in sea cucumber Apostichopus japonicus.
2014,
Pubmed
,
Echinobase
Zhang,
Characterization of a metalloprotease involved in Vibrio splendidus infection in the sea cucumber, Apostichopus japonicus.
2016,
Pubmed
,
Echinobase
Zhuang,
Cloning and characterization of the virulence factor Hop from Vibrio splendidus.
2020,
Pubmed
,
Echinobase
