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BACKGROUND: Doublecortin-like kinase1 and 2 (DCLKs) are protein Ser/Thr kinases important for neuronal development. More recently, they are also reported to regulate plasticity such as cell proliferation and differentiation of stem cells and cancer cells, but the details of their functions in this biological context are still unclear. With an attempt to reveal the functions of DCLKs in plasticity regulation, we here used the sea urchin embryo that undergoes highly regulative development as an experimental model.
RESULTS: We found that both the transcripts and the proteins of DCLKs are uniformly present during early embryogenesis and with some enrichment in mesenchymal cells after gastrula stage. Knockdown of DCLKs induced general developmental delay and defects at day 2. Further, the damage on the embryo/larva induced ectopic expression of DCLKs in the ectoderm where the damage was most severe. Under a tumor-prone or -suppressive condition, DCLKs expression was upregulated or downregulated, respectively, after damage. In both cases, the embryos showed severe developmental defects.
CONCLUSIONS: Taken together, a transient upregulation of DCLKs appears to be involved in a damage response both during normal and abnormal development, and which could result in different phenotypes in a context dependent manner.
Andrikou,
Myogenesis in the sea urchin embryo: the molecular fingerprint of the myoblast precursors.
2013, Pubmed,
Echinobase
Andrikou,
Myogenesis in the sea urchin embryo: the molecular fingerprint of the myoblast precursors.
2013,
Pubmed
,
Echinobase
Burgess,
Alternative splice variants of doublecortin-like kinase are differentially expressed and have different kinase activities.
2002,
Pubmed
Chandrakesan,
Dclk1, a tumor stem cell marker, regulates pro-survival signaling and self-renewal of intestinal tumor cells.
2017,
Pubmed
Deng,
Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2.
2011,
Pubmed
Fernandez-Nicolas,
A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin.
2019,
Pubmed
,
Echinobase
Gagnon,
Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
2014,
Pubmed
Gibson,
The origin of pigment cells in embryos of the sea urchin Strongylocentrotus purpuratus.
1985,
Pubmed
,
Echinobase
Hanahan,
Hallmarks of cancer: the next generation.
2011,
Pubmed
Koizumi,
DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons.
2017,
Pubmed
Nawabi,
Doublecortin-Like Kinases Promote Neuronal Survival and Induce Growth Cone Reformation via Distinct Mechanisms.
2015,
Pubmed
Patel,
Biochemical and Structural Insights into Doublecortin-like Kinase Domain 1.
2016,
Pubmed
Perillo,
Regulation of dynamic pigment cell states at single-cell resolution.
2020,
Pubmed
,
Echinobase
Schudrowitz,
Germline factor DDX4 functions in blood-derived cancer cell phenotypes.
2017,
Pubmed
Shevidi,
Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo.
2017,
Pubmed
,
Echinobase
Shin,
Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation.
2013,
Pubmed
Simpson,
Cancer/testis antigens, gametogenesis and cancer.
2005,
Pubmed
Westphalen,
Functional implication of Dclk1 and Dclk1-expressing cells in cancer.
2017,
Pubmed
Weygant,
Small molecule kinase inhibitor LRRK2-IN-1 demonstrates potent activity against colorectal and pancreatic cancer through inhibition of doublecortin-like kinase 1.
2014,
Pubmed
Yajima,
Ars insulator protects transgenes from long-term silencing in sea urchin larva.
2007,
Pubmed
,
Echinobase
Yajima,
Essential elements for translation: the germline factor Vasa functions broadly in somatic cells.
2015,
Pubmed
,
Echinobase
Yajima,
The DEAD-box RNA helicase Vasa functions in embryonic mitotic progression in the sea urchin.
2011,
Pubmed
,
Echinobase
Zygmunt,
Expression of alternatively spliced variants of the Dclk1 gene is regulated by psychotropic drugs.
2018,
Pubmed
