Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Anti-inflammatory and analgesic effects of the marine-derived compound comaparvin isolated from the crinoid Comanthus bennetti.
Chen LC, Lin YY, Jean YH, Lu Y, Chen WF, Yang SN, Wang HM, Jang IY, Chen IM, Su JH, Sung PJ, Sheu JH, Wen ZH.
???displayArticle.abstract???
To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.
Figure 1. Chemical structure and source of comaparvin. (A) Chemical structure of comaparvin. Molecular formula, C17H16O5; molecular weight, 300.11 Da; (B) The crinoid sample, Comanthus bennetti, was collected from Lamay Island, Taiwan.
Figure 3. Effect of comaparvin on the expression of the pro-inflammatory protein iNOS, in LPS-stimulated macrophage cells. (A) Western blot bands corresponding to the effects of comaparvin on iNOS and β-actin expression in LPS-stimulated macrophage cells; (B) The relative intensity of expression of iNOS protein in the LPS-alone group was set to 100%, and β-actin was used to verify that equivalent amounts of protein were loaded in each lane. Comaparvin significantly inhibited iNOS protein expression in LPS-stimulated macrophage cells. Data are the mean ± SEM values of 4 independent experiments. * p < 0.05, significant difference compared with the LPS-alone group.
Figure 8. Histopathologic analyses of the effects of comaparvin on cell infiltration in carrageen-injected paws. (A) Vehicle group (B) Carrageenan-alone group (C) Pre-treatment group (D) Post-treatment group. Pre-treatment or post-treatment of comaparvin (30 mg/kg) appeared to attenuate carrageenan-induced upregulation of immune cells. Numbers of neutrophils (E); macrophages (F); and fibroblasts (G) were analyzed in paw tissue from each group. Pre, comaparvin pre-treatment at 1 h before carrageenan injection. Post, comaparvin post-treatment at 4 h after carrageenan injection. Scale bar = 200 μm. Data are the mean ± SEM values. * p < 0.05, significant difference compared with the control group. #
p < 0.05, significant difference compared with the vehicle group.
Figure 9. Inhibitory effect of comaparvin on the expression of carrageenan-induced iNOS protein expression in rat paw tissue. Western blot analyses of expression of iNOS and β-actin protein in paw tissues after carrageenan injection. Intraplantar carrageenan injection induced significant iNOS protein expression in ipsilateral (carrageenan injection site) but not contralateral paw tissues at 24 h. Administration of a subcutaneous injection of comaparvin (30 mg/kg) significantly inhibited carrageenan-induced upregulation of iNOS protein expression. β-actin protein expression remained unchanged in all groups. Quantification data show the mean ± SEM values for four different experiments. Pre, comaparvin pre-treatment at 1 h before carrageenan injection. Post, comaparvin post-treatment at 4 h after carrageenan injection. Data are the mean ± SEM values. * p < 0.05 compared with the vehicle group. #
p < 0.05 compared with the carrageenan group.
Altemeier,
Fas (CD95) induces macrophage proinflammatory chemokine production via a MyD88-dependent, caspase-independent pathway.
2007, Pubmed
Altemeier,
Fas (CD95) induces macrophage proinflammatory chemokine production via a MyD88-dependent, caspase-independent pathway.
2007,
Pubmed Aneiros,
Bioactive peptides from marine sources: pharmacological properties and isolation procedures.
2004,
Pubmed Beck,
Inducible NO synthase: role in cellular signalling.
1999,
Pubmed Blunt,
Marine natural products.
2009,
Pubmed Bogdan,
Nitric oxide and the immune response.
2001,
Pubmed Bokesch,
Inhibition of ABCG2-mediated drug efflux by naphthopyrones from marine crinoids.
2010,
Pubmed
,
Echinobase Bolker,
Crinosterol: a unique sterol from a comatulid crinoid.
1967,
Pubmed
,
Echinobase Chaplan,
Quantitative assessment of tactile allodynia in the rat paw.
1994,
Pubmed Cheng,
Nitric oxide in cancer metastasis.
2014,
Pubmed Chovolou,
Identification of angular naphthopyrones from the Philippine echinoderm Comanthus species as inhibitors of the NF-κB signaling pathway.
2011,
Pubmed
,
Echinobase Coruzzi,
Antiinflammatory and antinociceptive effects of the selective histamine H4-receptor antagonists JNJ7777120 and VUF6002 in a rat model of carrageenan-induced acute inflammation.
2007,
Pubmed Cuzzocrea,
The role of the peroxisome proliferator-activated receptor-alpha (PPAR-alpha) in the regulation of acute inflammation.
2006,
Pubmed Dai,
Benzochromenones from the marine crinoid Comantheria rotula inhibit hypoxia-inducible factor-1 (HIF-1) in cell-based reporter assays and differentially suppress the growth of certain tumor cell lines.
2007,
Pubmed
,
Echinobase De Gois,
Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits.
2005,
Pubmed Ebada,
Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates.
2008,
Pubmed Folmer,
NF-kappaB-inhibiting naphthopyrones from the Fijian echinoderm Comanthus parvicirrus.
2008,
Pubmed
,
Echinobase García-Mediavilla,
The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells.
2007,
Pubmed Hargreaves,
A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.
1988,
Pubmed Ho,
The anti-inflammatory carbazole, LCY-2-CHO, inhibits lipopolysaccharide-induced inflammatory mediator expression through inhibition of the p38 mitogen-activated protein kinase signaling pathway in macrophages.
2004,
Pubmed Huang,
Sinularin from indigenous soft coral attenuates nociceptive responses and spinal neuroinflammation in carrageenan-induced inflammatory rat model.
2012,
Pubmed Jean,
Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory and analgesic effects of the natural marine compound lemnalol from Formosan soft coral Lemnalia cervicorni.
2008,
Pubmed Karin,
The IKK NF-kappa B system: a treasure trove for drug development.
2004,
Pubmed Korhonen,
Nitric oxide production and signaling in inflammation.
2005,
Pubmed Laille,
In vitro antiviral activity on dengue virus of marine natural products.
1998,
Pubmed Li,
NF-kappaB regulation in the immune system.
2002,
Pubmed Lin,
Intrathecal lemnalol, a natural marine compound obtained from Formosan soft coral, attenuates nociceptive responses and the activity of spinal glial cells in neuropathic rats.
2011,
Pubmed Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed Mayer,
Marine pharmacology in 2009-2011: marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems, and other miscellaneous mechanisms of action.
2013,
Pubmed Medzhitov,
Origin and physiological roles of inflammation.
2008,
Pubmed Montaser,
Marine natural products: a new wave of drugs?
2011,
Pubmed Park,
Repression of interferon-gamma-induced inducible nitric oxide synthase (iNOS) gene expression in microglia by sodium butyrate is mediated through specific inhibition of ERK signaling pathways.
2005,
Pubmed Radhika,
Anti-inflammatory activity of a new sphingosine derivative and cembrenoid diterpene (lobohedleolide) isolated from marine soft corals of Sinularia crassa TIXIER-DURIVAULT and Lobophytum species of the Andaman and Nicobar Islands.
2005,
Pubmed Ren,
Activation of adenosine A3 receptor alleviates TNF-α-induced inflammation through inhibition of the NF-κB signaling pathway in human colonic epithelial cells.
2014,
Pubmed Ricciotti,
Prostaglandins and inflammation.
2011,
Pubmed Salvemini,
Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation.
1996,
Pubmed Seibert,
Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain.
1994,
Pubmed Sekiguchi,
The potent inducible nitric oxide synthase inhibitor ONO-1714 inhibits neuronal NOS and exerts antinociception in rats.
2004,
Pubmed Senthilkumar,
Marine invertebrate natural products for anti-inflammatory and chronic diseases.
2013,
Pubmed Shao,
Bioactive constituents from the marine crinoid Himerometra magnipinna.
2007,
Pubmed
,
Echinobase Wang,
Stevioside plays an anti-inflammatory role by regulating the NF-κB and MAPK pathways in S. aureus-infected mouse mammary glands.
2014,
Pubmed Yen,
Role of acid-sensing ion channel 3 in sub-acute-phase inflammation.
2009,
Pubmed
