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J Cell Biol
1996 Oct 01;1351:191-9. doi: 10.1083/jcb.135.1.191.
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Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos.
Wilding M
,
Wright EM
,
Patel R
,
Ellis-Davies G
,
Whitaker M
.
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Using calcium-sensitive dyes together with their dextran conjugates and confocal microscopy, we have looked for evidence of localized calcium signaling in the region of the nucleus before entry into mitosis, using the sea urchin egg first mitotic cell cycle as a model. Global calcium transients that appear to originate from the nuclear area are often observed just before nuclear envelope breakdown (NEB). In the absence of global increases in calcium, confocal microscopy using Calcium Green-1 dextran indicator dye revealed localized calcium transients in the perinuclear region. We have also used a photoinactivatable calcium chelator, nitrophenyl EGTA (NP-EGTA), to test whether the chelator-induced block of mitosis entry can be reversed after inactivation of the chelator. Cells arrested before NEB by injection of NP-EGTA resume the cell cycle after flash photolysis of the chelator. Photolysis of chelator triggers calcium release. TreatmenT with caFfeine to enhance calcium-induced calcium release increases the amplitude of NEB-associated calcium transients. These results indicate that calcium increases local to the nucleus are required to trigger entry into mitosis. Local calcium transients arise in the perinuclear region and can spread from this region into the cytoplasm. Thus, cell cycle calcium signals are generated by the perinuclear mitotic machinery in early sea urchin embryos.
Allen,
Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.
1977, Pubmed
Allen,
Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.
1977,
Pubmed
Anraku,
Cell cycle control by calcium and calmodulin in Saccharomyces cerevisiae.
1991,
Pubmed
Baitinger,
Multifunctional Ca2+/calmodulin-dependent protein kinase is necessary for nuclear envelope breakdown.
1990,
Pubmed
,
Echinobase
Berger,
Ratio confocal imaging of free cytoplasmic calcium gradients in polarising and polarised Fucus zygotes.
1993,
Pubmed
Berridge,
Inositol trisphosphate and calcium signalling.
1993,
Pubmed
Brostrom,
Calcium-dependent regulation of protein synthesis in intact mammalian cells.
1990,
Pubmed
Cannell,
Spatial non-uniformities in [Ca2+]i during excitation-contraction coupling in cardiac myocytes.
1994,
Pubmed
Ciapa,
Cell-cycle calcium transients driven by cyclic changes in inositol trisphosphate levels.
1994,
Pubmed
,
Echinobase
Connor,
Intracellular calcium mobilization by inositol 1,4,5-trisphosphate: intracellular movements and compartmentalization.
1993,
Pubmed
Crossley,
Guanosine 5'-thiotriphosphate may stimulate phosphoinositide messenger production in sea urchin eggs by a different route than the fertilizing sperm.
1991,
Pubmed
,
Echinobase
Ellis-Davies,
Nitrophenyl-EGTA, a photolabile chelator that selectively binds Ca2+ with high affinity and releases it rapidly upon photolysis.
1994,
Pubmed
Endo,
Calcium release from the sarcoplasmic reticulum.
1977,
Pubmed
Endo,
Calcium induced release of calcium from the sarcoplasmic reticulum of skinned skeletal muscle fibres.
1970,
Pubmed
Fleischer,
Biochemistry and biophysics of excitation-contraction coupling.
1989,
Pubmed
Fluck,
Slow calcium waves accompany cytokinesis in medaka fish eggs.
1991,
Pubmed
Forer,
The role of the phosphatidylinositol cycle in mitosis in sea urchin zygotes. Lithium inhibition is overcome by myo-inositol but not by other cyclitols or sugars.
1987,
Pubmed
,
Echinobase
Gillot,
Calcium signals in and around the nucleus in sea urchin eggs.
1994,
Pubmed
,
Echinobase
Han,
Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.
1992,
Pubmed
Hepler,
Membranes in the mitotic apparatus: their structure and function.
1984,
Pubmed
Hepler,
Calcium and mitosis.
1992,
Pubmed
Hepler,
Free calcium increases during anaphase in stamen hair cells of Tradescantia.
1987,
Pubmed
Hepler,
Membranes in the mitotic apparatus of barley cells.
1980,
Pubmed
Iida,
Cell cycle control by Ca2+ in Saccharomyces cerevisiae.
1990,
Pubmed
Jones,
Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent.
1995,
Pubmed
Kao,
Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts.
1990,
Pubmed
Kasai,
Pancreatic calcium waves and secretion.
1995,
Pubmed
Keith,
Local cytoplasmic calcium gradients in living mitotic cells.
1985,
Pubmed
Kiehart,
Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system.
1981,
Pubmed
,
Echinobase
Kono,
A cell cycle-associated change in Ca2+ releasing activity leads to the generation of Ca2+ transients in mouse embryos during the first mitotic division.
1996,
Pubmed
Lechleiter,
Subcellular patterns of calcium release determined by G protein-specific residues of muscarinic receptors.
1991,
Pubmed
Lindsay,
Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts.
1995,
Pubmed
Lu,
Regulation of the cell cycle by calcium and calmodulin.
1993,
Pubmed
McDougall,
Function and characteristics of repetitive calcium waves associated with meiosis.
1995,
Pubmed
Meyer,
Regulation of nuclear calcium concentration.
1995,
Pubmed
Ohsumi,
Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae.
1983,
Pubmed
Ohya,
Yeast calmodulin: structural and functional elements essential for the cell cycle.
1992,
Pubmed
Patel,
Calcium-induced chromatin condensation and cyclin phosphorylation during chromatin condensation cycles in ammonia-activated sea urchin eggs.
1989,
Pubmed
,
Echinobase
Paweletz,
Membranes and microtubules of the mitotic apparatus of mammalian cells.
1981,
Pubmed
Petersen,
Local calcium spiking in pancreatic acinar cells.
1995,
Pubmed
Petzelt,
Visualization of the Ca-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.
1986,
Pubmed
,
Echinobase
Petzelt,
Localization of an intracellular membrane-bound Ca2+-ATPase in PtK-cells using immunofluorescence techniques.
1984,
Pubmed
Petzelt,
Biochemistry of the mitotic spindle.
1979,
Pubmed
Poenie,
Changes of free calcium levels with stages of the cell division cycle.
1985,
Pubmed
,
Echinobase
Poenie,
Calcium rises abruptly and briefly throughout the cell at the onset of anaphase.
1986,
Pubmed
Rasmussen,
Calcium as intracellular messenger: from simplicity to complexity.
1990,
Pubmed
Ratan,
Transition from metaphase to anaphase is accompanied by local changes in cytoplasmic free calcium in Pt K2 kidney epithelial cells.
1986,
Pubmed
Rizzuto,
Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria.
1993,
Pubmed
Silver,
Isolation of mitotic apparatus containing vesicles with calcium sequestration activity.
1980,
Pubmed
,
Echinobase
Steinhardt,
Intracellular free calcium and the first cell cycle of the sea-urchin embryo (Lytechinus pictus).
1990,
Pubmed
,
Echinobase
Steinhardt,
Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Stricker,
Time-lapse confocal imaging of calcium dynamics in starfish embryos.
1995,
Pubmed
,
Echinobase
Swann,
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs.
1986,
Pubmed
,
Echinobase
Terasaki,
Demonstration of calcium uptake and release by sea urchin egg cortical endoplasmic reticulum.
1991,
Pubmed
,
Echinobase
Terasaki,
Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.
1991,
Pubmed
,
Echinobase
Tombes,
Meiosis, egg activation, and nuclear envelope breakdown are differentially reliant on Ca2+, whereas germinal vesicle breakdown is Ca2+ independent in the mouse oocyte.
1992,
Pubmed
Twigg,
Translational control of InsP3-induced chromatin condensation during the early cell cycles of sea urchin embryos.
1988,
Pubmed
,
Echinobase
Whitaker,
Calcium and cell cycle control.
1990,
Pubmed
,
Echinobase
Whitaker,
Regulation of the cell division cycle by inositol trisphosphate and the calcium signaling pathway.
1995,
Pubmed
Wolniak,
Lithium alters mitotic progression in stamen hair cells of Tradescantia in a time-dependent and reversible fashion.
1987,
Pubmed
Wolniak,
The coincident distribution of calcium-rich membranes and kinetochore fibers at metaphase in living endosperm cells of Haemanthus.
1981,
Pubmed
Yao,
Quantal puffs of intracellular Ca2+ evoked by inositol trisphosphate in Xenopus oocytes.
1995,
Pubmed
