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J Cell Biol 1991 Nov 01;1154:1031-7. doi: 10.1083/jcb.115.4.1031.
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Demonstration of calcium uptake and release by sea urchin egg cortical endoplasmic reticulum.

Terasaki M , Sardet C .

The calcium indicator dye fluo-3/AM was loaded into the ER of isolated cortices of unfertilized eggs of the sea urchin Arbacia punctulata. Development of the fluorescent signal took from 8 to 40 min and usually required 1 mM ATP. The signal decreased to a minimum level within 30 s after perfusion with 1 microM InsP3 and increased within 5 min when InsP3 was replaced with 1 mM ATP. Also, the fluorescence signal was lowered rapidly by perfusion with 10 microM A23187 or 10 microM ionomycin. These findings demonstrate that the cortical ER is a site of ATP-dependent calcium sequestration and InsP3-induced calcium release. A light-induced wave of calcium release, traveling between 0.7 and 2.8 microns/s (average speed 1.4 microns/s, N = 8), was sometimes observed during time lapse recordings; it may therefore be possible to use the isolated cortex preparation to investigate the postfertilization calcium wave.

PubMed ID: 1955454
PMC ID: PMC2289945
Article link: J Cell Biol

Genes referenced: LOC100887844

References [+] :
Brady, Gelsolin inhibition of fast axonal transport indicates a requirement for actin microfilaments. 1984, Pubmed