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The expression of two zygotic genes (HE and BP10) during sea urchin embryogenesis was previously found to be early, transient, spatially restricted and controlled at the transcriptional level. Here we studied how the expression of these genes is affected when cell interactions are abolished by dissociating blastomeres and when development is perturbed by treatment with Li+. We found that in isolated blastomeres, transient transcriptional activity (HE) is unchanged and both genes apparently function in the appropriate cell type. Thus HE/BP10 expression is largely cell-autonomous and should rely on maternal factors unevenly distributed in the egg. Treatment with lithium does not affect the temporal control but decreases the transcriptional activity and the size of the domain of expression of the HE/BP10 genes. As the Li+ concentration increases, the border of the domain is progressively shifted towards the animal pole. This alteration of the spatial pattern is the earliest molecular evidence of a change in cell fate detectable only much later by morphological criteria, and reveals a gradient of sensitivity to Li+ along the animal--vegetal axis. These results suggest that the activity of the HE/BP10 genes is strongly dependent on spatially organized maternal information controlling early development.
Alexander,
Proteinases and extracellular matrix remodeling.
1989, Pubmed
Alexander,
Proteinases and extracellular matrix remodeling.
1989,
Pubmed
Angerer,
Molecular indices of cell lineage specification in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Berridge,
Neural and developmental actions of lithium: a unifying hypothesis.
1989,
Pubmed
Britten,
The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus.
1978,
Pubmed
,
Echinobase
Busa,
Lithium-induced teratogenesis in frog embryos prevented by a polyphosphoinositide cycle intermediate or a diacylglycerol analog.
1989,
Pubmed
Cameron,
The oral-aboral axis of a sea urchin embryo is specified by first cleavage.
1989,
Pubmed
,
Echinobase
Cameron,
Segregation of oral from aboral ectoderm precursors is completed at fifth cleavage in the embryogenesis of Strongylocentrotus purpuratus.
1990,
Pubmed
,
Echinobase
Cameron,
Lineage and fate of each blastomere of the eight-cell sea urchin embryo.
1987,
Pubmed
,
Echinobase
Davidson,
Spatial mechanisms of gene regulation in metazoan embryos.
1991,
Pubmed
Davidson,
Lineage-specific gene expression and the regulative capacities of the sea urchin embryo: a proposed mechanism.
1989,
Pubmed
,
Echinobase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Gagnon,
Posttranscriptional regulation of ectoderm-specific gene expression in early sea urchin embryos.
1992,
Pubmed
,
Echinobase
Gee,
The purification and properties of myo-inositol monophosphatase from bovine brain.
1988,
Pubmed
Giudice,
Reaggregation of dissociated cells of sea urchin embryos.
1970,
Pubmed
,
Echinobase
Goldstein,
Induction of gut in Caenorhabditis elegans embryos.
1992,
Pubmed
Gurdon,
Embryonic induction--molecular prospects.
1987,
Pubmed
Hall,
Hardening of the sea urchin fertilization envelope by peroxidase-catalyzed phenolic coupling of tyrosines.
1978,
Pubmed
,
Echinobase
Hallcher,
The effects of lithium ion and other agents on the activity of myo-inositol-1-phosphatase from bovine brain.
1980,
Pubmed
Hurley,
Altered expression of spatially regulated embryonic genes in the progeny of separated sea urchin blastomeres.
1989,
Pubmed
,
Echinobase
Johnson,
Cell interactions influence the fate of mouse blastomeres undergoing the transition from the 16- to the 32-cell stage.
1983,
Pubmed
Klein,
Lithium changes the ectodermal fate of individual frog blastomeres because it causes ectopic neural plate formation.
1989,
Pubmed
Labarca,
A simple, rapid, and sensitive DNA assay procedure.
1980,
Pubmed
Lepage,
Early expression of a collagenase-like hatching enzyme gene in the sea urchin embryo.
1990,
Pubmed
,
Echinobase
Lepage,
Purification and characterization of the sea urchin embryo hatching enzyme.
1989,
Pubmed
,
Echinobase
Lepage,
Spatial and temporal expression pattern during sea urchin embryogenesis of a gene coding for a protease homologous to the human protein BMP-1 and to the product of the Drosophila dorsal-ventral patterning gene tolloid.
1992,
Pubmed
,
Echinobase
Lepage,
Spatial expression of the hatching enzyme gene in the sea urchin embryo.
1992,
Pubmed
,
Echinobase
Livingston,
Lithium evokes expression of vegetal-specific molecules in the animal blastomeres of sea urchin embryos.
1989,
Pubmed
,
Echinobase
Livingston,
Range and stability of cell fate determination in isolated sea urchin blastomeres.
1990,
Pubmed
,
Echinobase
Maslanski,
Lithium-sensitive production of inositol phosphates during amphibian embryonic mesoderm induction.
1992,
Pubmed
Matrisian,
Metalloproteinases and their inhibitors in matrix remodeling.
1990,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Nemer,
Primary differentiation and ectoderm-specific gene expression in the animalized sea urchin embryo.
1985,
Pubmed
,
Echinobase
Nemer,
An altered series of ectodermal gene expressions accompanying the reversible suspension of differentiation in the zinc-animalized sea urchin embryo.
1986,
Pubmed
,
Echinobase
Nocente-McGrath,
Altered cell fate in LiCl-treated sea urchin embryos.
1991,
Pubmed
,
Echinobase
Nüsslein-Volhard,
Determination of the embryonic axes of Drosophila.
1991,
Pubmed
Parisi,
The pattern of cell division in the early development of the sea urchin. Paracentrotus lividus.
1978,
Pubmed
,
Echinobase
Peters,
Lithium ions induce prestalk-associated gene expression and inhibit prespore gene expression in Dictyostelium discoideum.
1989,
Pubmed
Reynolds,
Early mRNAs, spatially restricted along the animal-vegetal axis of sea urchin embryos, include one encoding a protein related to tolloid and BMP-1.
1992,
Pubmed
,
Echinobase
Shimell,
The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1.
1991,
Pubmed
Showman,
Removal of the fertilization membrane of sea urchin embryos employing aminotriazole.
1979,
Pubmed
,
Echinobase
Stephens,
Autonomous expression of tissue-specific genes in dissociated sea urchin embryos.
1989,
Pubmed
,
Echinobase
Wilt,
Determination and morphogenesis in the sea urchin embryo.
1987,
Pubmed
,
Echinobase
Wozney,
Novel regulators of bone formation: molecular clones and activities.
1988,
Pubmed
Yang,
Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.
1989,
Pubmed
,
Echinobase
