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Exp Cell Res
2010 Aug 01;31613:2204-11. doi: 10.1016/j.yexcr.2010.04.026.
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Use of specific glycosidases to probe cellular interactions in the sea urchin embryo.
Idoni B
,
Ghazarian H
,
Metzenberg S
,
Hutchins-Carroll V
,
Oppenheimer SB
,
Carroll EJ
.
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We present an unusual and novel model for initial investigations of a putative role for specifically conformed glycans in cellular interactions. We have used alpha- and ss-amylase and alpha- and ss-glucosidase in dose-response experiments evaluating their effects on archenteron organization using the NIH designated sea urchin embryo model. In quantitative dose-response experiments, we show that defined activity levels of alpha-glucosidase and ss-amylase inhibited archenteron organization in living Lytechinus pictus gastrula embryos, whereas all concentrations of ss-glucosidase and alpha-amylase were without substantial effects on development. Product inhibition studies suggested that the enzymes were acting by their specific glycosidase activities and polyacrylamide gel electrophoresis suggested that there was no detectable protease contamination in the active enzyme samples. The results provide evidence for a role of glycans in sea urchin embryo cellular interactions with special reference to the possible structural conformation of these glycans based on the differential activities of the alpha- and ss-glycosidases.
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20435035
???displayArticle.pmcLink???PMC2921930 ???displayArticle.link???Exp Cell Res ???displayArticle.grants???[+]
Asao,
Inhibition of cell aggregation by specific carbohydrates.
1979, Pubmed,
Echinobase
Asao,
Inhibition of cell aggregation by specific carbohydrates.
1979,
Pubmed
,
Echinobase
Davidson,
The sea urchin genome: where will it lead us?
2006,
Pubmed
,
Echinobase
Elödi,
Investigation of the active center of porcine-pancreatic amylase.
1972,
Pubmed
Evans,
Glycosyltrasferase activity in developing sea-urchin embryos.
1977,
Pubmed
,
Echinobase
Ghazarian,
Exogenous hyalin and sea urchin gastrulation. Part IV: a direct adhesion assay - progress in identifying hyalin's active sites.
2010,
Pubmed
,
Echinobase
Hsieh,
Partial purification and characterization of a soybean beta-glucosidase with high specific activity towards isoflavone conjugates.
2001,
Pubmed
Itza,
Septate junctions mediate the barrier to paracellular permeability in sea urchin embryos.
2005,
Pubmed
,
Echinobase
Khurrum,
Carbohydrate involvement in cellular interactions in sea urchin gastrulation.
2004,
Pubmed
,
Echinobase
Latham,
Accessing the embryo interior without microinjection.
1998,
Pubmed
,
Echinobase
Latham,
A putative role for carbohydrates in sea urchin gastrulation.
1999,
Pubmed
,
Echinobase
Oppenheimer,
Carbohydrate specificity of sea urchin blastula adhesion component.
1982,
Pubmed
,
Echinobase
Pili,
The alpha-glucosidase I inhibitor castanospermine alters endothelial cell glycosylation, prevents angiogenesis, and inhibits tumor growth.
1995,
Pubmed
Razinia,
Microplate assay for quantifying developmental morphologies: effects of exogenous hyalin on sea urchin gastrulation.
2007,
Pubmed
,
Echinobase
SMOGYI,
Notes on sugar determination.
1952,
Pubmed
Sajadi,
Cyclodextrin, a probe for studying adhesive interactions.
2007,
Pubmed
,
Echinobase
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
