Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1985 Jun 01;1006:1968-76. doi: 10.1083/jcb.100.6.1968.
Show Gene links
Show Anatomy links
The small subunit of ribonucleotide reductase is encoded by one of the most abundant translationally regulated maternal RNAs in clam and sea urchin eggs.
Standart NM
,
Bray SJ
,
George EL
,
Hunt T
,
Ruderman JV
.
???displayArticle.abstract???
In both clam oocytes and sea urchin eggs, fertilization triggers the synthesis of a set of proteins specified by stored maternal mRNAs. One of the most abundant of these (p41) has a molecular weight of 41,000. This paper describes the identification of p41 as the small subunit of ribonucleotide reductase, the enzyme that provides the precursors necessary for DNA synthesis. This identification is based mainly on the amino acid sequence deduced from cDNA clones corresponding to p41, which shows homology with a gene in Herpes Simplex virus that is thought to encode the small subunit of viral ribonucleotide reductase. Comparison with the B2 (small) subunit of Escherichia coli ribonucleotide reductase also shows striking homology in certain conserved regions of the molecule. However, our attention was originally drawn to protein p41 because it was specifically retained by an affinity column bearing the monoclonal antibody YL 1/2, which reacts with alpha-tubulin (Kilmartin, J. V., B. Wright, and C. Milstein, 1982, J. Cell Biol., 93:576-582). The finding that this antibody inhibits the activity of sea urchin embryo ribonucleotide reductase confirmed the identity of p41 as the small subunit. The unexpected binding of the small subunit of ribonucleotide reductase can be accounted for by its carboxy-terminal sequence, which matches the specificity requirements of YL 1/2 as determined by Wehland et al. (Wehland, J., H. C. Schroeder, and K. Weber, 1984, EMBO [Eur. Mol. Biol. Organ.] J., 3:1295-1300). Unlike the small subunit, there is no sign of synthesis of a corresponding large subunit of ribonucleotide reductase after fertilization. Since most enzymes of this type require two subunits for activity, we suspect that the unfertilized oocytes contain a stockpile of large subunits ready for combination with newly made small subunits. Thus, synthesis of the small subunit of ribonucleotide reductase represents a very clear example of the developmental regulation of enzyme activity by control of gene expression at the level of translation.
Alexandraki,
Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.
1985, Pubmed,
Echinobase
Alexandraki,
Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.
1985,
Pubmed
,
Echinobase
Anderson,
Processing of adenovirus 2-induced proteins.
1973,
Pubmed
Bacchetti,
Immunological characterization of herpes simplex virus type 1 and 2 polypeptide(s) involved in viral ribonucleotide reductase activity.
1984,
Pubmed
Biggin,
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
1983,
Pubmed
Brachet,
Effects of hydroxyurea on development and regeneration.
1967,
Pubmed
Carlson,
Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.
1984,
Pubmed
Cohen,
Ribonucleotide reductase activity of synchronized KB cells infected with herpes simplex virus.
1972,
Pubmed
Doolittle,
Similar amino acid sequences: chance or common ancestry?
1981,
Pubmed
Draper,
Detailed characterization of an apparently unspliced beta herpes simplex virus type 1 gene mapping in the interior of another.
1982,
Pubmed
Dutia,
Ribonucleotide reductase induced by herpes simplex virus has a virus-specified constituent.
1983,
Pubmed
Efstratiadis,
Enzymatic in vitro synthesis of globin genes.
1976,
Pubmed
Engström,
Ribonucleotide reductase from calf thymus. Purification and properties.
1979,
Pubmed
Eriksson,
Ribonucleotide reductase in cultured mouse lymphoma cells. Cell cycle-dependent variation in the activity of subunit protein M2.
1981,
Pubmed
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
,
Echinobase
Fansler,
Sea urchin nuclear DNA polymerase. II. Changing localization during early development.
1969,
Pubmed
,
Echinobase
Galloway,
Identification of proteins encoded by a fragment of herpes simplex virus type 2 DNA that has transforming activity.
1982,
Pubmed
Gibson,
Homology between two EBV early genes and HSV ribonucleotide reductase and 38K genes.
1984,
Pubmed
HULTIN,
The effect of puromycin on protein metabolism and cell division in fertilized sea urchin eggs.
1961,
Pubmed
,
Echinobase
Holmgren,
Regulation of ribonucleotide reductase.
1981,
Pubmed
Huszar,
Herpes simplex virus-induced ribonucleotide reductase: development of antibodies specific for the enzyme.
1983,
Pubmed
Huszar,
Partial purification and characterization of the ribonucleotide reductase induced by herpes simplex virus infection of mammalian cells.
1981,
Pubmed
Huszar,
Is ribonucleotide reductase the transforming function of herpes simplex virus 2?
1983,
Pubmed
Kilmartin,
Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
1982,
Pubmed
Kozak,
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
1983,
Pubmed
Kuhn,
Chromatin proteins of sea urchin embryos: dual origin from an oogenetic reservoir and new synthesis.
1981,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Mabuchi,
Purification and properties of soluble actin from sea urchin eggs.
1980,
Pubmed
,
Echinobase
Mathews,
Giant pools of DNA precursors in sea urchin eggs.
1975,
Pubmed
,
Echinobase
McLauchlan,
DNA sequence homology between two co-linear loci on the HSV genome which have different transforming abilities.
1983,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Noronha,
Induction of a reductive pathway for deoxyribonucleotide synthesis during early embryogenesis of the sea urchin.
1972,
Pubmed
,
Echinobase
Ponstingl,
Complete amino acid sequence of alpha-tubulin from porcine brain.
1981,
Pubmed
Racine,
DNA polymerase alpha and beta in the California urchin.
1978,
Pubmed
,
Echinobase
Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed
Rosenthal,
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
1983,
Pubmed
Rosenthal,
Selective translation of mRNA controls the pattern of protein synthesis during early development of the surf clam, Spisula solidissima.
1980,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schiff,
Promotion of microtubule assembly in vitro by taxol.
1979,
Pubmed
Staden,
An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
1982,
Pubmed
Tansey,
Differential mRNA accumulation and translation during Spisula development.
1983,
Pubmed
Thelander,
Reduction of ribonucleotides.
1979,
Pubmed
Timourian,
Protein synthesis requirements for the first two cleavages in sea urchin eggs.
1967,
Pubmed
,
Echinobase
Vallee,
A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs).
1982,
Pubmed
Wagenaar,
The timing of synthesis of proteins required for mitosis in the cell cycle of the sea urchin embryo.
1983,
Pubmed
,
Echinobase
Wehland,
A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.
1983,
Pubmed
Wehland,
Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
1984,
Pubmed
Young,
Synthesis of protein for DNA replication and cleavage events in the sand dollar embryo.
1969,
Pubmed
,
Echinobase
de Petrocellis,
Enzymes of DNA biosynthesis in developing sea urchins. Changes in ribonucleotide reductase, thymidine, and thymidylate kinase activities.
1976,
Pubmed
,
Echinobase
