Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nucleic Acids Res
1988 Jan 11;161:179-98. doi: 10.1093/nar/16.1.179.
Show Gene links
Show Anatomy links
Sequence and properties of the message encoding Tetrahymena hv1, a highly evolutionarily conserved histone H2A variant that is associated with active genes.
White EM
,
Shapiro DL
,
Allis CD
,
Gorovsky MA
.
???displayArticle.abstract???
hv1 is a histone H2A variant found in the transcriptionally active Tetrahymena macronucleus, but not in the transcriptionally inert micronucleus. hv1 also contains antigenic determinants conserved in the histone complements of representatives of all four eukaryotic kingdoms. A cDNA clone encoding hv1 has been isolated and sequenced. Comparison of the derived protein sequence of hv1 with that of the chicken variant H2A.F and the sea urchin variant H2A.F/Z reveals remarkable homology in all but the extreme amino- and carboxy-termini and a small region in the conserved core. Putative regions of conserved antigenicity are discussed. Evidence is presented that suggests that hv1 is a single-copy, intron-containing gene that encodes a polyadenylated message. Unusual features in the 3'' flanking sequence and in codon usage are also described. Evidence is also presented showing that hv1 message amounts are ten-fold greater in growing cells than in starved cells.
Allis,
hv1 is an evolutionarily conserved H2A variant that is preferentially associated with active genes.
1986, Pubmed
Allis,
hv1 is an evolutionarily conserved H2A variant that is preferentially associated with active genes.
1986,
Pubmed
Allis,
Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, Tetrahymena thermophila.
1980,
Pubmed
Allis,
A conserved histone variant enriched in nucleoli of mammalian cells.
1982,
Pubmed
Ball,
Amino acid sequence of the N-terminal domain of calf thymus histone H2A.Z.
1983,
Pubmed
Bannon,
Tetrahymena H4 genes: structure, evolution and organization in macro- and micronuclei.
1984,
Pubmed
Bannon,
Multiple, independently regulated, polyadenylated messages for histone H3 and H4 in Tetrahymena.
1983,
Pubmed
Bhatnagar,
Immunocytochemical localization of a histone H2A variant in the mammalian nucleolar chromatin.
1984,
Pubmed
Blackburn,
Genomic reorganization in ciliated protozoans.
1986,
Pubmed
Brown,
Changes in the levels of three different classes of histone mRNA during murine erythroleukemia cell differentiation.
1985,
Pubmed
Brush,
Replacement variant histone genes contain intervening sequences.
1985,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
D'Andrea,
Vertebrate histone genes: nucleotide sequence of a chicken H2A gene and regulatory flanking sequences.
1981,
Pubmed
,
Echinobase
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Ernst,
Characterization of a cDNA clone coding for a sea urchin histone H2A variant related to the H2A.F/Z histone protein in vertebrates.
1987,
Pubmed
,
Echinobase
Fahrner,
Yeast histone mRNA is polyadenylated.
1980,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Fusauchi,
Tetrahymena histone H2A. Isolation and two variant sequences.
1983,
Pubmed
Gorovsky,
Isolation of micro- and macronuclei of Tetrahymena pyriformis.
1975,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Harvey,
H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.
1983,
Pubmed
Hayashi,
Tetrahymena histone H4. Complete amino acid sequences of two variants.
1984,
Pubmed
Helms,
A new method for purifying lambda DNA from phage lysates.
1985,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Horowitz,
Unusual features of transcribed and translated regions of the histone H4 gene family of Tetrahymena thermophila.
1987,
Pubmed
Howard,
Reproducible and variable genomic rearrangements occur in the developing somatic nucleus of the ciliate Tetrahymena thermophila.
1985,
Pubmed
Ikemura,
Codon usage and tRNA content in unicellular and multicellular organisms.
1985,
Pubmed
Isenberg,
Histones.
1979,
Pubmed
Kolodrubetz,
Histone H2A subtypes associate interchangeably in vivo with histone H2B subtypes.
1982,
Pubmed
Nickel,
Changes in the histone H2A variant H2A.Z and polyubiquitinated histone species in developing trout testis.
1987,
Pubmed
Nomoto,
Characterization of two types of histone H2B genes from macronuclei of Tetrahymena thermophila.
1987,
Pubmed
Okayama,
High-efficiency cloning of full-length cDNA.
1982,
Pubmed
Old,
Histone genes: not so simple after all.
1984,
Pubmed
Pederson,
Changes in chromatin structure accompany modulation of the rate of transcription of 5S ribosomal genes in Tetrahymena.
1984,
Pubmed
Pustell,
A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination.
1984,
Pubmed
Rykowski,
Histone H2B subtypes are dispensable during the yeast cell cycle.
1981,
Pubmed
Sogin,
Evolutionary diversity of eukaryotic small-subunit rRNA genes.
1986,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sures,
The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
1978,
Pubmed
,
Echinobase
Wenkert,
Timing of the appearance of macronuclear-specific histone variant hv1 and gene expression in developing new macronuclei of Tetrahymena thermophila.
1984,
Pubmed
Woudt,
The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences.
1983,
Pubmed
,
Echinobase
Wu,
Histones and their modifications.
1986,
Pubmed
Wu,
An intervening sequence in an unusual histone H1 gene of Tetrahymena thermophila.
1986,
Pubmed
Wu,
Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.
1981,
Pubmed
Yu,
In situ dot blots: quantitation of mRNA in intact cells.
1986,
Pubmed
