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Simultaneous localization of myosin and tubulin in human tissue culture cells by double antibody staining.
Fujiwara K
,
Pollard TD
.
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We have studied the distribution of myosin and tubulin molecules inside the same tissue culture cells by using two antibodies labeled with contrasting fluorochromes. Antimyosin raised against human platelet myosin was labeled with rhodamine. Antitubulin raised against sea urchin vinblastine-induced tubulin crystals was labeled with fluorescein. The two antibodies stained entirely different structures inside the same flat interphase cell: antimyosin bound to stress fibers and antitubulin bound to thin, wavy fibers thought to be individual microtubules. Compact interphase cells stained diffusely with both antibodies. From prophase through early anaphase both antibodies stained the mitotic spindle, although the fluorescence contrast between the spindle and the cytoplasm was much higher with antitubulin than with antimyosin. From anaphase through telophase, strong antimyosin staining occurred in the cleavage furrow, while antitubulin stained the region between the separated chromosomes. This study established the feasibility of high-resolution fluorescent antibody localization of pairs of motility proteins in the cytoplasm of single cells, an approach which will make it possible to map out the sites of the various contractile protein interactions in situ.
Bensch,
Microtubular crystals in mammalian cells.
1969, Pubmed
Bensch,
Microtubular crystals in mammalian cells.
1969,
Pubmed
Brinkley,
Cytoplasmic microtubules in normal and transformed cells in culture: analysis by tubulin antibody immunofluorescence.
1975,
Pubmed
Bryan,
Vinblastine and microtubules. I. Induction and isolation of crystals from sea urchin oocytes.
1971,
Pubmed
,
Echinobase
Bryan,
Vinblastine and microtubules. II. Characterization of two protein subunits from the isolated crystals.
1972,
Pubmed
,
Echinobase
Cebra,
Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains.
1965,
Pubmed
de Petris,
Concanavalin A receptors, immunoglobulins, and theta antigen of the lymphocyte surface. Interactions with concanavalin A and with Cytoplasmic structures.
1975,
Pubmed
Frye,
The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons.
1970,
Pubmed
Fujiwara,
Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells.
1976,
Pubmed
Goldstein,
Immunofluorescent detection of human antibodies reactive with tumor.
1971,
Pubmed
Ishikawa,
Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.
1969,
Pubmed
Lazarides,
Actin antibody: the specific visualization of actin filaments in non-muscle cells.
1974,
Pubmed
Munthe,
Detection of genetic markers of human immunoglobulins by immunofluorescence technique.
1971,
Pubmed
Schreiner,
Redistribution of myosin accompanying capping of surface Ig.
1977,
Pubmed
Strahs,
Potentiation of vinblastine crystal formation in vivo by puromycin and colcemid.
1973,
Pubmed
,
Echinobase
Weber,
Antibody against tuberlin: the specific visualization of cytoplasmic microtubules in tissue culture cells.
1975,
Pubmed
,
Echinobase
Weber,
Antibody to myosin: the specific visualization of myosin-containing filaments in nonmuscle cells.
1974,
Pubmed
Weber,
Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.
1975,
Pubmed
