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Proc Natl Acad Sci U S A 1975 Feb 01;722:459-63. doi: 10.1073/pnas.72.2.459.
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Antibody against tuberlin: the specific visualization of cytoplasmic microtubules in tissue culture cells.

Weber K , Pollack R , Bibring T .

Cytoplasmic microtubules in tissue culture cells can be directly visualized by immunofluorescence microscopy. Antibody against tubulin from the outer doublets of sea urchin sperm flagella decorates a network of fine cytoplasmic fibers in a variety of cell lines of human, monkey, rat, mouse, and chicken origin. These fibers are separate and of uniform thickness and are seen throughout the cytoplasm. The fibers disappear either in a medium containing colchicine or after subjection of the cells to low temperature. The same treatments do not destroy the microfilamentous structures that are visualized by means of antibody against actin. When tryspin-treated enucleated cells are replated and then stained with antibody against tubulin, the fibers can be seen to traverse the entire enucleated cytoplasm.

PubMed ID: 804694
PMC ID: PMC432331
Article link: Proc Natl Acad Sci U S A

Genes referenced: LOC100887844 LOC100893907 LOC115919910 LOC590297 tubgcp2

References [+] :
Behnke, Evidence for four classes of microtubules in individual cells. 1967, Pubmed