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Elife 2018 May 01;7. doi: 10.7554/eLife.36600.
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A SLC4 family bicarbonate transporter is critical for intracellular pH regulation and biomineralization in sea urchin embryos.

Hu MY , Yan JJ , Petersen I , Himmerkus N , Bleich M , Stumpp M .

Efficient pH regulation is a fundamental requisite of all calcifying systems in animals and plants but with the underlying pH regulatory mechanisms remaining largely unknown. Using the sea urchin larva, this work identified the SLC4 HCO3- transporter family member SpSlc4a10 to be critically involved in the formation of an elaborate calcitic endoskeleton. SpSlc4a10 is specifically expressed by calcifying primary mesenchyme cells with peak expression during de novo formation of the skeleton. Knock-down of SpSlc4a10 led to pH regulatory defects accompanied by decreased calcification rates and skeleton deformations. Reductions in seawater pH, resembling ocean acidification scenarios, led to an increase in SpSlc4a10 expression suggesting a compensatory mechanism in place to maintain calcification rates. We propose a first pH regulatory and HCO3- concentrating mechanism that is fundamentally linked to the biological precipitation of CaCO3. This knowledge will help understanding biomineralization strategies in animals and their interaction with a changing environment.

PubMed ID: 29714685
PMC ID: PMC5986267
Article link: Elife

Species referenced: Echinodermata
Genes referenced: irak1bp1 LOC100887844 LOC115919910 LOC580912 LOC581395 LOC590371 mos NBC SpZ12-1
Antibodies: LOC580912 Ab1
Morpholinos: LOC580912 MO1

Article Images: [+] show captions
References [+] :
Anderson, Matrix vesicles and calcification. 2003, Pubmed