Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis.
Srayko M
,
Buster DW
,
Bazirgan OA
,
McNally FJ
,
Mains PE
.
???displayArticle.abstract???
The Caenorhabditis elegans meiotic spindle is morphologically distinct from the first mitotic spindle, yet both structures form in the same cytoplasm approximately 20 minutes apart. The mei-1 and mei-2 genes of C. elegans are required for the establishment of the oocyte meiotic spindle but are not required for mitotic spindle function. mei-1 encodes an AAA ATPase family member with similarity to the p60 catalytic subunit of the heterodimeric sea urchin microtubule-severing protein, katanin. We report that mei-2 encodes a 280-amino acid protein containing a region similar to the p80-targeting subunit of katanin. MEI-1 and MEI-2 antibodies decorate the polar ends of meiotic spindle microtubules and meiotic chromatin. We find that the subcellular location of MEI-2 depends on wild-type mei-1 activity and vice versa. These experiments, combined with MEI-1 and MEI-2''s similarity to p60 and p80 katanin, suggest that the C. elegans proteins function as a complex. In support of this idea, MEI-1 and MEI-2 physically associate in HeLa cells. Furthermore, co-expression of MEI-1 and MEI-2 in HeLa cells results in the disassembly of microtubules. These data lead us to conclude that MEI-1/MEI-2 microtubule-severing activity is required for meiotic spindle organization in C. elegans.
Afshar,
DNA binding and meiotic chromosomal localization of the Drosophila nod kinesin-like protein.
1995, Pubmed
Afshar,
DNA binding and meiotic chromosomal localization of the Drosophila nod kinesin-like protein.
1995,
Pubmed
Ahmad,
An essential role for katanin in severing microtubules in the neuron.
1999,
Pubmed
Albertson,
Formation of the first cleavage spindle in nematode embryos.
1984,
Pubmed
Albertson,
Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans.
1993,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Austin,
glp-1 is required in the germ line for regulation of the decision between mitosis and meiosis in C. elegans.
1987,
Pubmed
Babst,
Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p.
1997,
Pubmed
Barton,
Gain-of-function mutations of fem-3, a sex-determination gene in Caenorhabditis elegans.
1987,
Pubmed
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
C. elegans Sequencing Consortium,
Genome sequence of the nematode C. elegans: a platform for investigating biology.
1998,
Pubmed
Clandinin,
Genetic studies of mei-1 gene activity during the transition from meiosis to mitosis in Caenorhabditis elegans.
1993,
Pubmed
Clark-Maguire,
Localization of the mei-1 gene product of Caenorhaditis elegans, a meiotic-specific spindle component.
1994,
Pubmed
Clark-Maguire,
mei-1, a gene required for meiotic spindle formation in Caenorhabditis elegans, is a member of a family of ATPases.
1994,
Pubmed
Cleveland,
Unpolymerized tubulin modulates the level of tubulin mRNAs.
1981,
Pubmed
Dow,
Genetic and molecular characterization of the caenorhabditis elegans gene, mel-26, a postmeiotic negative regulator of mei-1, a meiotic-specific spindle component.
1998,
Pubmed
Fire,
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
1998,
Pubmed
Fire,
Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle.
1991,
Pubmed
Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
Guo,
par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed.
1995,
Pubmed
Hartman,
Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit.
1998,
Pubmed
,
Echinobase
Huang,
A second trans-spliced RNA leader sequence in the nematode Caenorhabditis elegans.
1989,
Pubmed
Kelly,
Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans gene.
1997,
Pubmed
Kemphues,
Two loci required for cytoplasmic organization in early embryos of Caenorhabditis elegans.
1986,
Pubmed
Kirschner,
Beyond self-assembly: from microtubules to morphogenesis.
1986,
Pubmed
Krause,
A trans-spliced leader sequence on actin mRNA in C. elegans.
1987,
Pubmed
Lohret,
A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation.
1998,
Pubmed
Mains,
Mutations affecting the meiotic and mitotic divisions of the early Caenorhabditis elegans embryo.
1990,
Pubmed
Matthews,
ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.
1998,
Pubmed
Matthies,
Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein.
1996,
Pubmed
McKim,
Chromosome I duplications in Caenorhabditis elegans.
1990,
Pubmed
McNally,
Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin.
2000,
Pubmed
McNally,
Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.
1996,
Pubmed
,
Echinobase
McNally,
Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs.
1998,
Pubmed
McNally,
Identification of katanin, an ATPase that severs and disassembles stable microtubules.
1993,
Pubmed
,
Echinobase
Mello,
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
1991,
Pubmed
Merdes,
Pathways of spindle pole formation: different mechanisms; conserved components.
1997,
Pubmed
Meuwissen,
A coiled-coil related protein specific for synapsed regions of meiotic prophase chromosomes.
1992,
Pubmed
Montgomery,
RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans.
1998,
Pubmed
Nelson,
Intersex, a temperature-sensitive mutant of the nematode Caenorhabditis elegans.
1978,
Pubmed
Patel,
The AAA team: related ATPases with diverse functions.
1998,
Pubmed
Piperno,
Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms.
1985,
Pubmed
,
Echinobase
Sawada,
Microtubules in ascidian eggs during meiosis, fertilization, and mitosis.
1988,
Pubmed
Schatten,
The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization.
1994,
Pubmed
Strome,
Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos.
1983,
Pubmed
Theurkauf,
Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein.
1992,
Pubmed
Tournebize,
Role of chromosomes in assembly of meiotic and mitotic spindles.
1997,
Pubmed
Vale,
Severing of stable microtubules by a mitotically activated protein in Xenopus egg extracts.
1991,
Pubmed
Vernos,
Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning.
1995,
Pubmed
Walczak,
A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity.
1998,
Pubmed
Wang,
Chromokinesin: a DNA-binding, kinesin-like nuclear protein.
1995,
Pubmed
Yen,
CENP-E is a putative kinetochore motor that accumulates just before mitosis.
1992,
Pubmed
