Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1992 Jul 01;8913:6001-5. doi: 10.1073/pnas.89.13.6001.
Show Gene links
Show Anatomy links
Early persistent activation of sperm K+ channels by the egg peptide speract.
Babcock DF
,
Bosma MM
,
Battaglia DE
,
Darszon A
.
???displayArticle.abstract???
Transduction by sperm of the instructive signal provided by the egg peptide speract involves rapid, complex changes in internal ion and cyclic nucleotide content. Here, investigations of hypotonically swollen sperm provide insight into the underlying processes and identify K+ channel activation as an initial ionic event in gamete recognition. A sustained hyperpolarization of swollen sperm is promoted by less than 2.5 pM speract and is followed (with greater than 100 pM speract) by transient repolarization and (with greater than 10 nM speract) by depolarization that is dependent on external Ca2+. Monophasic increases in pHi are produced only by greater than 25 pM speract, indicating that hyperpolarization may not directly promote alkalinization. Increased K(+)-selective (K+ greater than Rb+ greater than Cs+ greater than Na+) membrane permeability is found after all speract greater than 2.5 pM, suggesting that hyperpolarization results from persistent activation of K+ channels and that repolarization has a different ionic basis. Supporting this contention, the K+ channel blocker tetraethylammonium (20 mM) inhibits the increased K+ permeability that follows treatment of swollen sperm (and of sperm in seawater) with 2.5 pM speract. Such induced activation of K+ channels is observed in patch-clamped swollen sperm examined in the cell-attached configuration, upon application of 5-50 pM speract to the bath medium. The efficacy of externally applied speract and its potency indicate that activation is indirect and probably involves an as yet unidentified diffusible mediator whose production is promoted by speract at concentrations 0.01-0.001 times those predicted from reported estimates of the Kd for the known speract receptor.
Babcock,
Independent elevation of cytosolic [Ca2+] and pH of mammalian sperm by voltage-dependent and pH-sensitive mechanisms.
1987, Pubmed
Babcock,
Independent elevation of cytosolic [Ca2+] and pH of mammalian sperm by voltage-dependent and pH-sensitive mechanisms.
1987,
Pubmed
Babcock,
Examination of the intracellular ionic environment and of ionophore action by null point measurements employing the fluorescein chromophore.
1983,
Pubmed
Bentley,
Receptor-mediated activation of detergent-solubilized guanylate cyclase.
1988,
Pubmed
,
Echinobase
Boulay,
The human N-formylpeptide receptor. Characterization of two cDNA isolates and evidence for a new subfamily of G-protein-coupled receptors.
1990,
Pubmed
Dangott,
Cloning of the mRNA for the protein that crosslinks to the egg peptide speract.
1989,
Pubmed
,
Echinobase
Dangott,
Identification and partial characterization of the receptor for speract.
1984,
Pubmed
,
Echinobase
Garbers,
The amino acid sequence and chemical synthesis of speract and of speract analogues.
1982,
Pubmed
,
Echinobase
Garbers,
Molecular basis of fertilization.
1989,
Pubmed
García-Soto,
Internal pH can regulate Ca2+ uptake and the acrosome reaction in sea urchin sperm.
1987,
Pubmed
,
Echinobase
Guerrero,
Single-channel activity in sea urchin sperm revealed by the patch-clamp technique.
1987,
Pubmed
,
Echinobase
Hansbrough,
Speract. Purification and characterization of a peptide associated with eggs that activates spermatozoa.
1981,
Pubmed
,
Echinobase
Hansbrough,
Sodium-dependent activation of sea urchin spermatozoa by speract and monensin.
1981,
Pubmed
,
Echinobase
Harvath,
Oxidized N-formylmethionyl-leucyl-phenylalanine: effect on the activation of human monocyte and neutrophil chemotaxis and superoxide production.
1984,
Pubmed
Heytler,
Uncouplers of oxidative phosphorylation.
1979,
Pubmed
Hoffman,
Determination of membrane potentials in human and Amphiuma red blood cells by means of fluorescent probe.
1974,
Pubmed
Lazzari,
Neutrophil hyperpolarization in response to a chemotactic peptide.
1990,
Pubmed
Lee,
Internal GTP stimulates the speract receptor mediated voltage changes in sea urchin spermatozoa membrane vesicles.
1988,
Pubmed
,
Echinobase
Lee,
Modulation of the voltage-sensitive Na+/H+ exchange in sea urchin spermatozoa through membrane potential changes induced by the egg peptide speract.
1986,
Pubmed
,
Echinobase
Lievano,
Single-channel activity of bilayers derived from sea urchin sperm plasma membranes at the tip of a patch-clamp electrode.
1985,
Pubmed
,
Echinobase
Murphy,
Cloning of complementary DNA encoding a functional human interleukin-8 receptor.
1991,
Pubmed
Ramarao,
A single mRNA encodes multiple copies of the egg peptide speract.
1990,
Pubmed
,
Echinobase
Repaske,
A hydrogen ion flux mediates stimulation of respiratory activity by speract in sea urchin spermatozoa.
1983,
Pubmed
,
Echinobase
Schackmann,
Measurement of plasma membrane and mitochondrial potentials in sea urchin sperm. Changes upon activation and induction of the acrosome reaction.
1984,
Pubmed
,
Echinobase
Schackmann,
Alteration of intracellular [Ca2+] in sea urchin sperm by the egg peptide speract. Evidence that increased intracellular Ca2+ is coupled to Na+ entry and increased intracellular pH.
1986,
Pubmed
,
Echinobase
Schulz,
The primary structure of a plasma membrane guanylate cyclase demonstrates diversity within this new receptor family.
1989,
Pubmed
Shimomura,
Covalent coupling of a resact analogue to guanylate cyclase.
1986,
Pubmed
,
Echinobase
Suzuki,
Some more speract derivatives associated with eggs of sea urchins, Pseudocentrotus depressus, Strongylocentrotus purpuratus, Hemicentrotus pulcherrimus and Anthocidaris crassispina.
1988,
Pubmed
,
Echinobase
Suzuki,
Stimulation of sperm respiration rates by speract and resact at alkaline extracellular pH.
1984,
Pubmed
,
Echinobase
Suzuki,
Purification and the primary structure of sperm-activity peptides from the jelly coat of sea urchin eggs.
1981,
Pubmed
,
Echinobase
Tilney,
The polymerization of actin. II. How nonfilamentous actin becomes nonrandomly distributed in sperm: evidence for the association of this actin with membranes.
1976,
Pubmed
Ward,
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer.
1985,
Pubmed
,
Echinobase
