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The foxa regulatory gene is of central importance for endoderm specification across Bilateria, and this gene lies at an essential node of the well-characterized sea urchin endomesoderm gene regulatory network (GRN). Here we experimentally dissect the cis-regulatory system that controls the complex pattern of foxa expression in these embryos. Four separate cis-regulatory modules (CRMs) cooperate to control foxa expression in different spatial domains of the endomesoderm, and at different times. A detailed mutational analysis revealed the inputs to each of these cis-regulatory modules. The complex and dynamic expression of foxa is regulated by a combination of repressors, a permissive switch, and multiple activators. A mathematical kinetic model was applied to study the dynamic response of foxa cis-regulatory modules to transient inputs. This study shed light on the mesoderm-endoderm fate decision and provides a functional explanation, in terms of the genomic regulatory code, for the spatial and temporal expression of a key developmental control gene.
Arenas-Mena,
Hindgut specification and cell-adhesion functions of Sphox11/13b in the endoderm of the sea urchin embryo.
2006, Pubmed,
Echinobase
Arenas-Mena,
Hindgut specification and cell-adhesion functions of Sphox11/13b in the endoderm of the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Ben-Tabou de-Leon,
Modeling the dynamics of transcriptional gene regulatory networks for animal development.
2009,
Pubmed
Bolouri,
Transcriptional regulatory cascades in development: initial rates, not steady state, determine network kinetics.
2003,
Pubmed
,
Echinobase
Boyle,
Developmental expression of foxA and gata genes during gut formation in the polychaete annelid, Capitella sp. I.
2008,
Pubmed
Brown,
Paircomp, FamilyRelationsII and Cartwheel: tools for interspecific sequence comparison.
2005,
Pubmed
Burtscher,
Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo.
2009,
Pubmed
Croce,
Dynamics of Delta/Notch signaling on endomesoderm segregation in the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Friedman,
The Foxa family of transcription factors in development and metabolism.
2006,
Pubmed
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Hiruta,
Comparative expression analysis of transcription factor genes in the endostyle of invertebrate chordates.
2005,
Pubmed
Hong,
Shadow enhancers as a source of evolutionary novelty.
2008,
Pubmed
Kim,
Repressor activity of Headless/Tcf3 is essential for vertebrate head formation.
2000,
Pubmed
Lee,
Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo.
2007,
Pubmed
,
Echinobase
Livant,
Differential stability of expression of similarly specified endogenous and exogenous genes in the sea urchin embryo.
1991,
Pubmed
,
Echinobase
Logan,
Nuclear beta-catenin is required to specify vegetal cell fates in the sea urchin embryo.
1999,
Pubmed
,
Echinobase
Minokawa,
cis-Regulatory inputs of the wnt8 gene in the sea urchin endomesoderm network.
2005,
Pubmed
,
Echinobase
Nam,
Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network.
2007,
Pubmed
,
Echinobase
Oliveri,
Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Peter,
The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage.
2010,
Pubmed
,
Echinobase
Range,
LvGroucho and nuclear beta-catenin functionally compete for Tcf binding to influence activation of the endomesoderm gene regulatory network in the sea urchin embryo.
2005,
Pubmed
,
Echinobase
Rast,
Transgenic manipulation of the sea urchin embryo.
2000,
Pubmed
,
Echinobase
Revilla-i-Domingo,
R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres.
2004,
Pubmed
,
Echinobase
Röttinger,
Nemo-like kinase (NLK) acts downstream of Notch/Delta signalling to downregulate TCF during mesoderm induction in the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Smith,
A gene regulatory network subcircuit drives a dynamic pattern of gene expression.
2007,
Pubmed
,
Echinobase
Suri,
Inhibition of mesodermal fate by Xenopus HNF3beta/FoxA2.
2004,
Pubmed
Sweet,
LvDelta is a mesoderm-inducing signal in the sea urchin embryo and can endow blastomeres with organizer-like properties.
2002,
Pubmed
,
Echinobase
Wahl,
The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo.
2009,
Pubmed
,
Echinobase
Weitzel,
Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled.
2004,
Pubmed
,
Echinobase
Wikramanayake,
Nuclear beta-catenin-dependent Wnt8 signaling in vegetal cells of the early sea urchin embryo regulates gastrulation and differentiation of endoderm and mesodermal cell lineages.
2004,
Pubmed
,
Echinobase
