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Summary Attributions Wiki
XB-GRNA-25900325
gRNA Name: add1 gRNA1
Synonyms: Xt.abra gRNA
Genomic Target: add1

Sequence: 5' GATTCTATTGCGTACGAAAA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 19/20 19/20
genome
gRNA
 
5' TTTTCGTACGCAATAGAATC 3'
   ||||||||||||||||||||
3' AAAAGCATGCGTTATCTTAG 5'
 
5' TTTTCGTAAGCAATAGAATC 3'
   |||||||| |||||||||||
3' AAAAGCATGCGTTATCTTAG 5'
 
5' TTTTCATACGCAATAGAATC 3'
   ||||| ||||||||||||||
3' AAAAGCATGCGTTATCTTAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr1L:23097545..23097564 19/20 Sense target add1.L
Chr1S:13414941..13414960 19/20 Sense target add1.S
Chr1L:173976362..173976381 18/20 Antisense unknown
Chr1S:99810729..99810748 16/20 Antisense unknown
tropicalis 10.0 Chr1:22075541..22075560 20/20 Sense target add1
Chr5:47954000..47954019 16/20 Sense off-target crim1
Chr3:42016315..42016334 17/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022     
???morpholino.displaySummary.papersRecent??? Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis., Dev Cell 2025     
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