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XB-ART-61394
Methods Mol Biol 2025 Jan 01;2923:63-74. doi: 10.1007/978-1-0716-4522-2_5.
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HiChIP to Study 3D Genome Organization in Xenopus tropicalis Embryos.

Hussein AO , Blitz IL , Cho KWY .


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During zygotic genome activation, the embryonic genome starts to organize into three-dimensional (3D) structures such as topologically associated domains and chromatin loops, which are essential for regulating gene expression during development. HiChIP is a powerful tool for investigating 3D genome organization by identifying proteins associated with long-range chromatin interactions. In this chapter, we provide a protocol for preparing HiChIP libraries from Xenopus tropicalis embryos.

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