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Mar Drugs
2020 Jul 18;187:. doi: 10.3390/md18070370.
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Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells.
Li J
,
Li Y
,
Li Y
,
Yang Z
,
Jin H
.
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Collagen is a promising biomaterial used in the beauty and biomedical industries. In this study, the physicochemical characterization, antioxidant activities, and protective effects against H2O2-induced injury of collagen isolated from Acaudina molpadioides were investigated. The amino acid composition analysis showed that the collagen was rich in glycine (Gly), alanine (Ala), and glutamic acid (Glu), but poor in tyrosine (Tyr) and phenylalanine (Phe). Zeta potential analysis revealed that the isoelectric point (pI) of collagen from Acaudina molpadioides was about 4.25. It possessed moderate scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals in a dose-dependent manner. In addition, the collagen was able to effectively improve cell viability and morphology, inhibit the production of Malondialdehyde (MDA), and increase the activities of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) in cultured RAW264.7 cells, resulting in a protective effect against H2O2-induced injury. Overall, the results showed that collagen extracted from A. molpadioides has promising prospects in the beauty and cosmetics industries.
LQ20D060004 Natural Science Foundation of Zhejiang Province, 2019JZ00005 Fundamental Research Funds for the Provincial Universities of Zhejiang Province
Figure 1. Ultraviolet (UV) spectra of collagen from the body wall of A. molpadioides.
Figure 2. The fourier transform infrared spectroscopy (FTIR) spectrum of collagen from the body wall of A. molpadioides.
Figure 3. Zeta potential of collagen from A. molpadioides at different pH values.
Figure 4. The scavenging activities of 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) at different concentrations of collagen from A. molpadioides. (a–g) values with different letters indicated significant differences in the same samples at different concentrations (p < 0.05); (A–B) values with different letters indicated significant differences in the different samples at the same concentration (p < 0.05).
Figure 5. Effect of collagen from A. molpadioides on H2O2-induced injury RAW264.7 cells viability. The cell viability was measured after incubation for 24 h in medium with or without collagen and exposed to H2O2 (1 mM) for 4 h. Values with different letters were significantly different (p < 0.05).
Figure 6. Effect of collagen from A. molpadioides on H2O2-induced injury RAW264.7 cell morphology. The cells of each group were directly observed under an inverted microscope, and the background of the pictures were set to green. (A) control group; (B) model group (1 mM H2O2); (C) 6.25 µg/mL collagen + 1 mM H2O2; (D) 12.5 µg/mL collagen + 1 mM H2O2; (E) 25.0 µg/mL collagen + 1 mM H2O2; (F) 50.0 µg/mL collagen + 1 mM H2O2.
Figure 7. Effect of collagen from A. molpadioides on levels of Malondialdehyde (MDA) (A), Superoxide Dismutase (SOD) (B), and Glutathione Peroxidase (GSH-Px) (C) in RAW264.7 cells induced by H2O2. Values with different letters were significantly different (p < 0.05).
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