Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Cells Dev
2021 Mar 01;165:203660. doi: 10.1016/j.cdev.2021.203660.
Show Gene links
Show Anatomy links
Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking.
Remsburg C
,
Testa M
,
Song JL
.
???displayArticle.abstract???
Rab35 is a small GTPase that regulates plasma membrane to early endosome vesicular trafficking and mediates actin remodeling to form actin-rich cellular structures. While the function of Rab35 in the cellular context has been examined, its role during development has not been well studied. In this study, we take advantage of the sea urchin's high fecundity, external fertilization, and transparent embryos to determine the function of Rab35 during development. We found that loss of function of Rab35 results in defects in skeletogenesis and gastrulation, which were rescued by co-injection of sea urchin Rab35. The loss of Rab35's function results in decreased endocytosis and impaired exocytosis, which may be important for skeletogenesis and gastrulation. Skeletal spicules of Rab35 knockdown embryos have reduced organized actin compared to the control, supporting the notion that Rab35 regulates actin dynamics. In addition, the skeletal and gastrulation defects induced by Rab35 knockdown were rescued by co-injection with Fascin, an actin-bundling protein, indicating that proper actin dynamics play a critical role for both skeletogenesis and gastrulation. Overall, results indicate that through its role in mediating vesicular trafficking and actin remodeling, Rab35 is an important regulator of embryonic structure formation in early development.
???displayArticle.pubmedLink???
33842922
???displayArticle.pmcLink???PMC8028849 ???displayArticle.link???Cells Dev ???displayArticle.grants???[+]
Adomako-Ankomah,
P58-A and P58-B: novel proteins that mediate skeletogenesis in the sea urchin embryo.
2011, Pubmed,
Echinobase
Adomako-Ankomah,
P58-A and P58-B: novel proteins that mediate skeletogenesis in the sea urchin embryo.
2011,
Pubmed
,
Echinobase
Allaire,
The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes.
2010,
Pubmed
Allaire,
Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration.
2013,
Pubmed
Alvares,
Expression of the invertebrate sea urchin P16 protein into mammalian MC3T3 osteoblasts transforms and reprograms them into "osteocyte-like" cells.
2016,
Pubmed
,
Echinobase
Annunziata,
Pattern and process during sea urchin gut morphogenesis: the regulatory landscape.
2014,
Pubmed
,
Echinobase
Anstrom,
Sea urchin primary mesenchyme cells: ingression occurs independent of microtubules.
1989,
Pubmed
,
Echinobase
Anstrom,
Microfilaments, cell shape changes, and the formation of primary mesenchyme in sea urchin embryos.
1992,
Pubmed
,
Echinobase
Beane,
RhoA regulates initiation of invagination, but not convergent extension, during sea urchin gastrulation.
2006,
Pubmed
,
Echinobase
Beghein,
Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function.
2018,
Pubmed
Biesemann,
Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.
2017,
Pubmed
Bonder,
Actin-membrane cytoskeletal dynamics in early sea urchin development.
1995,
Pubmed
,
Echinobase
Brumback,
Using FM1-43 to study neuropeptide granule dynamics and exocytosis.
2004,
Pubmed
Cameron,
SpBase: the sea urchin genome database and web site.
2009,
Pubmed
,
Echinobase
Chaineau,
Rab35: GEFs, GAPs and effectors.
2013,
Pubmed
Charrasse,
Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion.
2013,
Pubmed
Cheers,
Rapid microinjection of fertilized eggs.
2004,
Pubmed
,
Echinobase
Cheers,
P16 is an essential regulator of skeletogenesis in the sea urchin embryo.
2005,
Pubmed
,
Echinobase
Chesneau,
An ARF6/Rab35 GTPase cascade for endocytic recycling and successful cytokinesis.
2012,
Pubmed
Chua,
Rab35--a vesicular traffic-regulating small GTPase with actin modulating roles.
2010,
Pubmed
Clancy,
Coordinated Regulation of Intracellular Fascin Distribution Governs Tumor Microvesicle Release and Invasive Cell Capacity.
2019,
Pubmed
Davidson,
How do sea urchins invaginate? Using biomechanics to distinguish between mechanisms of primary invagination.
1995,
Pubmed
,
Echinobase
Decker,
Skeletogenesis in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Di Tommaso,
T-Coffee: a web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension.
2011,
Pubmed
Dikshit,
Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells.
2015,
Pubmed
Duloquin,
Localized VEGF signaling from ectoderm to mesenchyme cells controls morphogenesis of the sea urchin embryo skeleton.
2007,
Pubmed
,
Echinobase
Dutta,
Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.
2015,
Pubmed
Ettensohn,
The regulation of primary mesenchyme cell patterning.
1990,
Pubmed
,
Echinobase
Ettensohn,
The evolution of a new cell type was associated with competition for a signaling ligand.
2019,
Pubmed
,
Echinobase
Grant,
Pathways and mechanisms of endocytic recycling.
2009,
Pubmed
Guzmán-Hernández,
Secretion of VEGF-165 has unique characteristics, including shedding from the plasma membrane.
2014,
Pubmed
Hashimoto,
The roles of fascins in health and disease.
2011,
Pubmed
Jayo,
Fascin: a key regulator of cytoskeletal dynamics.
2010,
Pubmed
Jewett,
Planar polarized Rab35 functions as an oscillatory ratchet during cell intercalation in the Drosophila epithelium.
2017,
Pubmed
Kimberly,
Bottle cells are required for the initiation of primary invagination in the sea urchin embryo.
1998,
Pubmed
,
Echinobase
Klinkert,
Rab35 GTPase: A Central Regulator of Phosphoinositides and F-actin in Endocytic Recycling and Beyond.
2016,
Pubmed
Klinkert,
Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening.
2016,
Pubmed
Kominami,
Gastrulation in the sea urchin embryo: a model system for analyzing the morphogenesis of a monolayered epithelium.
2004,
Pubmed
,
Echinobase
Kouranti,
Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis.
2006,
Pubmed
Kudtarkar,
Echinobase: an expanding resource for echinoderm genomic information.
2017,
Pubmed
Kuo,
Effector Specificity Mechanisms of Rab35 GTPase.
2019,
Pubmed
Marat,
Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton.
2012,
Pubmed
McClay,
Evolutionary crossroads in developmental biology: sea urchins.
2011,
Pubmed
,
Echinobase
McClay,
Gastrulation in the sea urchin.
2020,
Pubmed
,
Echinobase
Miller,
Characterization of the role of cadherin in regulating cell adhesion during sea urchin development.
1997,
Pubmed
,
Echinobase
Morgulis,
Possible cooption of a VEGF-driven tubulogenesis program for biomineralization in echinoderms.
2019,
Pubmed
,
Echinobase
Nakajima,
The initial phase of gastrulation in sea urchins is accompanied by the formation of bottle cells.
1996,
Pubmed
,
Echinobase
Nozumi,
Coordinated Movement of Vesicles and Actin Bundles during Nerve Growth Revealed by Superresolution Microscopy.
2017,
Pubmed
Pereira-Leal,
Evolution of the Rab family of small GTP-binding proteins.
2001,
Pubmed
Rafiq,
The genomic regulatory control of skeletal morphogenesis in the sea urchin.
2012,
Pubmed
,
Echinobase
Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed
Sato,
Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein.
2008,
Pubmed
Schneider,
NIH Image to ImageJ: 25 years of image analysis.
2012,
Pubmed
Sepúlveda-Ramírez,
Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo.
2018,
Pubmed
,
Echinobase
Sethi,
Multicolor labeling in developmental gene regulatory network analysis.
2014,
Pubmed
,
Echinobase
Shim,
Rab35 mediates transport of Cdc42 and Rac1 to the plasma membrane during phagocytosis.
2010,
Pubmed
Stepicheva,
High throughput microinjections of sea urchin zygotes.
2014,
Pubmed
,
Echinobase
Stepicheva,
The small GTPase Arf6 regulates sea urchin morphogenesis.
2017,
Pubmed
,
Echinobase
Stumpp,
Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification.
2012,
Pubmed
,
Echinobase
Takata,
Pigment cells trigger the onset of gastrulation in tropical sea urchin Echinometra mathaei.
2004,
Pubmed
,
Echinobase
Terasaki,
Labeling of cell membranes and compartments for live cell fluorescence microscopy.
2004,
Pubmed
Tu,
Quantitative developmental transcriptomes of the sea urchin Strongylocentrotus purpuratus.
2014,
Pubmed
,
Echinobase
Uytterhoeven,
Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins.
2011,
Pubmed
Vidavsky,
Calcium transport into the cells of the sea urchin larva in relation to spicule formation.
2016,
Pubmed
,
Echinobase
Wu,
Ingression of primary mesenchyme cells of the sea urchin embryo: a precisely timed epithelial mesenchymal transition.
2007,
Pubmed
,
Echinobase
Yajima,
Autonomy in specification of primordial germ cells and their passive translocation in the sea urchin.
2012,
Pubmed
,
Echinobase
Zhang,
Rab35 controls actin bundling by recruiting fascin as an effector protein.
2009,
Pubmed
