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ECB-ART-49057
Zygote 2022 Apr 01;302:213-216. doi: 10.1017/S0967199421000514.
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Time-dependent expression of ryanodine receptors in sea urchin eggs, zygotes and early embryos.

Percivale G , Angelini C , Falugi C , Picco C , Prestipino G .


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In this work, the presence of calcium-dependent calcium channels and their receptors (RyR) has been investigated in Paracentrotus lividus eggs and early embryos, from unfertilized egg to four-blastomere stages. Electrophysiological recordings of RyR single-channel current fluctuations showed that RyRs are functional during the first developmental events with a maximum at zygote stage, c. 40 min after fertilization, corresponding to the first cleavage. The nature of vertebrate-like RyRs active at this stage was established by specific activation/blockade experiments.

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