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Nanomaterials (Basel)
2020 Feb 23;102:. doi: 10.3390/nano10020390.
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Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo.
Diniz FR
,
Maia RCAP
,
Rannier L
,
Andrade LN
,
V Chaud M
,
da Silva CF
,
Corrêa CB
,
de Albuquerque Junior RLC
,
P da Costa L
,
Shin SR
,
Hassan S
,
Sanchez-Lopez E
,
Souto EB
,
Severino P
.
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Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430-450 nm in the ultraviolet-visible (UV-Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT-IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm-1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.
M-ERA-NET/0004/2015 (PAIRED) and UIDB/04469/2020 Portuguese Science and Technology Foundation (FCT), CNPq, #443238/2014-6, #470388/2014-5 Coordenação Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC), Conselho Nacional de Desenvolvimento Científico e Tecnológico
Figure 1. Ultraviolet-visible (UV–Vis) absorption spectra of sodium alginate, gelatin, and hydrogel with silver nanoparticles at different concentrations (1 mM, 2 mM, and 4 mM).
Figure 2. Fourier Transform Infra-red (FT–IR) spectra of (A) sodium alginate, (B) gelatin, and of hydrogels with silver nanoparticles at different concentrations (C) 1 mM, (D) 2 mM, and (E) 4 mM. Arrows of spectrum A correspond to the bands around 1650 cm−1 (C=C stretching), 1250 cm−1 (C–O–C stretching); arrows of spectrum B correspond to 1640 cm−1 and 1510 cm−1 of amide carbonyl (C=O and C=N stretching vibration); spectra of the hydrogel (square of C, D, and E) are the bands around 1600–1500 cm−1.
Figure 3. Differential Scanning Calorimetry (DSC) analysis (left-hand panel) of the (A) hydrogel alginate/gelatin (80:20) and (B) hydrogel with silver nanoparticles (AgNPs) (4 mM); Thermogravimetric (TGA) analysis (right-hand panel) of (A) hydrogel alginate/gelatin (80:20), and (B) hydrogel with AgNPs (4 mM).
Figure 4. Electron micrographs of the hydrogels with silver nanoparticles at different concentrations: 1 mM on the 200 nm scale (a) and 50 nm scale (b); 4 mM on the 200 nm (c) and 100 nm scale (d).
Figure 5. Cell viability assay of gelatin, hydrogel containing 4 mM of AgNPs, sodium alginate, and silver nitrate of human L929 fibroblasts, determined by the methyl-thiazolyl-tetrazolium (MTT) assay after 24 h of incubation. The vehicle used to dilute the drug (dimethyl sulfoxide, DMSO 5%) was used as the negative control (100% viability). The data correspond to the mean ± SEM of four independent experiments. * p < 0.05 compared to the control group using one-way analysis of variance followed by Tukey’s test.
Figure 6. Non-splinted model showing the percentage of the non-epithelialized surface of the wound of the groups: GCTR (Control Group), GH (Group with hydrogel sodium alginate/gelatin (80:20), and GHP (Group hydrogel with AgNP 4 mM AgNO3). All values are mean ± S.E. Statistical analysis comprised ANOVA followed by Tukey’s test. * P < 0.05 in relation to GCTR, GH, and GHP groups, respectively (n = 21/group).
Figure 7. Photomicrographs of hematoxylin/eosin-stained histological sections representative of histological wound healing versus the time course of the experiment. Day 3: Wounds present intense edema (ed) and infiltration of polymorphonuclear neutrophils; note the lymphocyte-rich infiltrate (lym) and immature granulation tissue (igt) in the bottom of GH and GHP, respectively (100×). Polymorphonuclear neutrophil (small lobular nuclei) and lymphocytes (dark round nuclei) are highlighted in higher magnification (800×). Day 7: Thick strips of granulation tissue are observed in all groups (dashed arrows); irregular and slit-shaped capillary blood vessels concentrated in the edge are seen in GCTR (thin arrows), whereas dilated hyperemic vessels (thick arrows) are observed throughout the wound areas in GH and GHP (100×). Note the lower content of inflammatory cells in GHP. Stromal spindle cells (fibroblast and endothelial-like cells) are highlighted at higher magnification (800×). Day 14: Residual vascular granulation tissue (right) is observed in GCTR, but a cellular primary fibrous scar (cfb) is seen in GH and GHP. Epithelial buddings (compatible with rudimentary cutaneous appendages) (white arrows) are found in the edges of the wound area in GH but over the full epithelial surface in GHP (100×). Stromal spindle cells (fibroblast-like cells) are highlighted in higher magnification (800×). GCTR (Control Group), GH (Group with hydrogel sodium alginate/gelatin (80:20), and GHP (Group hydrogel with AgNP 4 mM AgNO3).
Aderibigbe,
Alginate in Wound Dressings.
2018, Pubmed
Aderibigbe,
Alginate in Wound Dressings.
2018,
Pubmed
Annamalai,
Green synthesis of silver nanoparticles: characterization and determination of antibacterial potency.
2016,
Pubmed
Ashmore,
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles.
2018,
Pubmed
Ashour,
Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties.
2015,
Pubmed
Barreto,
Improvement of wound tissue repair by chitosan films containing (-)-borneol, a bicyclic monoterpene alcohol, in rats.
2016,
Pubmed
Bianconi,
n-3 polyunsaturated fatty acids for the prevention of arrhythmia recurrence after electrical cardioversion of chronic persistent atrial fibrillation: a randomized, double-blind, multicentre study.
2011,
Pubmed
Blasi,
Lipid nanoparticles for drug delivery to the brain: in vivo veritas.
2009,
Pubmed
Debone,
Chitosan/Copaiba oleoresin films for would dressing application.
2019,
Pubmed
,
Echinobase
Gunasekaran,
Silver nanoparticles as real topical bullets for wound healing.
2011,
Pubmed
Gómez Chabala,
Release Behavior and Antibacterial Activity of Chitosan/Alginate Blends with Aloe vera and Silver Nanoparticles.
2017,
Pubmed
Hussain,
Recent Advances in Polymer-based Wound Dressings for the Treatment of Diabetic Foot Ulcer: An Overview of State-of-the-art.
2018,
Pubmed
Ilkar Erdagi,
Genipin crosslinked gelatin-diosgenin-nanocellulose hydrogels for potential wound dressing and healing applications.
2020,
Pubmed
Iravani,
Synthesis of silver nanoparticles: chemical, physical and biological methods.
2014,
Pubmed
Kamoun,
A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings.
2017,
Pubmed
Kang,
Biosynthesis of gold and silver chloride nanoparticles mediated by Crataegus pinnatifida fruit extract: in vitro study of anti-inflammatory activities.
2018,
Pubmed
Kanmani,
Physicochemical properties of gelatin/silver nanoparticle antimicrobial composite films.
2014,
Pubmed
Kumar,
Cellular imaging and bactericidal mechanism of green-synthesized silver nanoparticles against human pathogenic bacteria.
2018,
Pubmed
Lazar,
Quorum sensing in biofilms--how to destroy the bacterial citadels or their cohesion/power?
2011,
Pubmed
Liu,
Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing.
2010,
Pubmed
Lustosa,
In Situ Synthesis of Silver Nanoparticles in a Hydrogel of Carboxymethyl Cellulose with Phthalated-Cashew Gum as a Promising Antibacterial and Healing Agent.
2017,
Pubmed
Mattyasovszky,
Cytokine Interferon-γ suppresses the function of capsule myofibroblasts and induces cell apoptosis.
2017,
Pubmed
Mekkawy,
In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels.
2017,
Pubmed
Mogoşanu,
Natural and synthetic polymers for wounds and burns dressing.
2014,
Pubmed
Pandey,
Sodium alginate stabilized silver nanoparticles-silica nanohybrid and their antibacterial characteristics.
2016,
Pubmed
Peppas,
Hydrogels in pharmaceutical formulations.
2000,
Pubmed
Periasamy,
How Staphylococcus aureus biofilms develop their characteristic structure.
2012,
Pubmed
Rath,
Collagen nanofiber containing silver nanoparticles for improved wound-healing applications.
2016,
Pubmed
Rescignano,
PVA bio-nanocomposites: a new take-off using cellulose nanocrystals and PLGA nanoparticles.
2014,
Pubmed
Rigon,
Solid lipid nanoparticles optimized by 22 factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts.
2018,
Pubmed
Salehi,
The Therapeutic Potential of Apigenin.
2019,
Pubmed
Severino,
Alginate Nanoparticles for Drug Delivery and Targeting.
2019,
Pubmed
,
Echinobase
Severino,
Sodium alginate-cross-linked polymyxin B sulphate-loaded solid lipid nanoparticles: Antibiotic resistance tests and HaCat and NIH/3T3 cell viability studies.
2015,
Pubmed
Shen,
Interferon-gamma inhibits healing post scald burn injury.
2012,
Pubmed
Smart,
Assessment of Minimum Inhibitory Concentrations of Telavancin by Revised Broth Microdilution Method in Phase 3 Hospital-Acquired Pneumonia/Ventilator-Associated Pneumonia Clinical Isolates.
2016,
Pubmed
Souto,
New Nanotechnologies for the Treatment and Repair of Skin Burns Infections.
2020,
Pubmed
Souto,
Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines.
2020,
Pubmed
Stojkovska,
Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments.
2018,
Pubmed
Sánchez-López,
Metal-Based Nanoparticles as Antimicrobial Agents: An Overview.
2020,
Pubmed
Tarusha,
Alginate membranes loaded with hyaluronic acid and silver nanoparticles to foster tissue healing and to control bacterial contamination of non-healing wounds.
2018,
Pubmed
Tian,
Topical delivery of silver nanoparticles promotes wound healing.
2007,
Pubmed
Trøstrup,
Uncontrolled gelatin degradation in non-healing chronic wounds.
2018,
Pubmed
Tyliszczak,
Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles.
2017,
Pubmed
WINTER,
Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig.
1962,
Pubmed
Winter,
Formation of the scab and the rate of epithelisation of superficial wounds in the skin of the young domestic pig. 1962.
1995,
Pubmed
Xu,
Carboxymethyl chitosan/gelatin/hyaluronic acid blended-membranes as epithelia transplanting scaffold for corneal wound healing.
2018,
Pubmed
Ye,
In situ reduction of silver nanoparticles by gelatin to obtain porous silver nanoparticle/chitosan composites with enhanced antimicrobial and wound-healing activity.
2019,
Pubmed
Zheng,
Gelatin-Based Hydrogels Blended with Gellan as an Injectable Wound Dressing.
2018,
Pubmed
Zhou,
Simultaneous silencing of TGF-β1 and COX-2 reduces human skin hypertrophic scar through activation of fibroblast apoptosis.
2017,
Pubmed