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Methods Mol Biol January 1, 2019; 1834 193-207.

Generation and Analysis of Xenopus laevis Models of Retinal Degeneration Using CRISPR/Cas9.

Feehan JM , Stanar P , Tam BM , Chiu C , Moritz OL .

Xenopus laevis have proven to be a useful system for rapid generation and analysis of transgenic models of human retinal disease. However, experimental approaches in this system were limited by lack of a robust knockdown or knockout technology. Here we describe a protocol for generation of Cas9-edited X. laevis embryos. The technique introduces point mutations into the genome of X. laevis resulting in in-frame and out-of-frame insertions and deletions that allow modeling of both dominant and recessive human diseases and efficiently generates gene knockdown and knockout. Our techniques can produce high-frequency gene editing in X. laevis, permitting analysis in the F0 generation.

PubMed ID: 30324446
Article link: Methods Mol Biol

Genes referenced: clspn LOC574887