Hissae Yassue-Cordeiro P et al. (2019), Development of Chitosan/Silver Sulfadiazine/Zeo...

ECB-ART-47489

Pharmaceutics 2019 Oct 14;1110:. doi: 10.3390/pharmaceutics11100535.

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Development of Chitosan/Silver Sulfadiazine/Zeolite Composite Films for Wound Dressing.

Hissae Yassue-Cordeiro P , Zandonai CH , Pereira Genesi B , Santos Lopes P , Sanchez-Lopez E , Garcia ML , Camargo Fernandes-Machado NR , Severino P , B Souto E , Ferreira da Silva C .

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Biopolymeric films with silver sulfadiazine (AgSD) are proposed as an alternative to the occlusive AgSD-containing creams and gauzes, which are commonly used in the treatment of conventional burns. While the recognized cytotoxicity of AgSD has been reported to compromise its use as an antimicrobial drug in pharmaceuticals, this limitation can be overcome by developing sustained-release formulations. Microporous materials as zeolites can be used as drug delivery systems for sustained release of AgSD. The purpose of this work was the development and characterization of chitosan/zeolite composite films to be used as wound dressings. Zeolite was impregnated with AgSD before the production of the composite films. The physicochemical properties of zeolites and the films were evaluated, as well as the antimicrobial activity of the polymeric films and the cytotoxicity of the films in fibroblasts Balb 3T3/c. Impregnated zeolite exhibited changes in FTIR spectra and XRD diffraction patterns, in comparison to non-impregnated composites, which corroborate the results obtained with EDX-SEM. The pure chitosan film was compact and without noticeable defects and macropores, while the film with zeolite was opaquer, more rigid, and efficient against Candida albicans and some gram-negative bacteria. The safety evaluation showed that although the AgSD films present cytotoxicity, they could be used in a concentration-dependent fashion.

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Genes referenced: LOC115919910 LOC576508

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References [+] :

Abdel-Fattah, Tailoring the properties and functions of phosphate/silk/Ag/chitosan scaffolds. 2015, Pubmed

	Figure 1. Component performed by X-ray spectrometer probe (EDX) for the zeolite AgSD-Y. (a) original image; (b) Al; (c) Si; (d) O; (e) Na; (f) Ag.
	Figure 2. SEM micrograph of AgSD-Y zeolite (magnification of 2400×).
	Figure 3. Diffractograms of AgSD-Y zeolite.
	Figure 4. Heating curve of differential scanning calorimetry (DSC) of (a) pure AgSD and (b) NaY and AgSD-Y zeolite.
	Figure 5. Curve of AgSD, AgSD-Y and NaY samples.
	Figure 6. Spectrum of (a) NaY, AgSD-Y zeolite and (b) pure AgSD.
	Figure 7. SEM micrographs of chitosan film (left: surface at magnification 500×); right: cross-section at magnification 3000×): AgSD/chitosan film (a,b); AgSD-Y/Chitosan film (c,d).
	Figure 8. Diffractograms of (a) chitosan films, chitosan powder and (b) AgSD-Y powder, AgSD-Y/chitosan film and AgSD/chitosan film.
	Figure 9. Heating curves of Chitosan films, AgSD/Chitosan film, and AgSD-Y/Chitosan film.
	Figure 10. Curve for the Chitosan film and AgSD/Chitosan film and AgSD-Y/Chitosan film.
	Figure 11. Infrared spectrum of the chitosan film, AgSD-Y/Chitosan film and AgSD/Chitosan film.
	Figure 12. Release-test from AgSD/Chitosan film and AgSD-Y/Chitosan film.
	Figure 13. Tests (cell viability of S. aureous, E. coli, P. aeruginosa and C. albicans) in liquid medium containing AgSD-Y/Chitosan film, AgSD/Chitosan film, Chitosan film or AgNO3. Results are the mean ± standard deviation (n = 3).
	Figure 14. Effect of extract concentration on growth inhibition of Balb/c 3T3 fibroblasts with AgSD/Chitosan film and AgSD-Y/Chitosan film.