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In Vitro and in Vivo Scolicidal Activities of Holothuria leucospilota Extract and CeO2 Nanoparticles against Hydatid Cyst.
Aryamand S
,
Khademvatan S
,
Hazrati Tappeh K
,
Heshmatian B
,
Jelodar A
.
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Background: We aimed to investigate the scolicidal effects of Holothuria leucospilota extract and CeO2 nanoparticles against protoscoleces of hydatid cysts in-vitro and in-vivo.
Methods: Hydatid cysts were collected from, Urmia slaughterhouses between years 2016-2017 and the hydatid fluid aspirated from the fertile cysts. Various concentration of H. leucospilota extract, CeO2 NPs and combination of CeO2-NPs/H. leucospilota were used for 10-60 min to evaluate the viability of protoscoleces by 0.1% eosin method. CASPASE -3 activity measured for assessment of cell apoptosis in treated protoscoleces. BALB/c mice were infected intraperitoneally with 2000 viable protoscoleces and treated daily for 4 wk by intragastrical inoculation with H. leucospilota, CeO2 NPs, combination of CeO2 NPs/H. leucospilota and Albendazole. Cyst development was macroscopically analyzed.
Results: H. leucospilota extract and combination of CeO2 NPs/H. leucospilota have potent scolicidal activity at concentration of 20 mg/ml and 15 mg/ml after 60 min treatment. Maximum caspase-3 activity was observed when protoscoleces expose with H. leucospilota and combination H. leucospilota & CeO2 NPs. After treatment of cyst infected mice with extract and CeO2 NPs, combination of CeO2 NPs/H leucospilota and albendazole, a significant decrease in number of cysts, size and volume of cyst (P<0.05) was observed.
Conclusion: This result shows an antihydatic and scolicidal effects of H. leucospilota extract and CeO2 NPs.
Fig. 1:. Dead protoscoleces of E. granulosus after exposure with 0.1% eosin
Fig. 2:. Live protoscoleces of E. granulosus after exposure with 0.1% eosin
Fig. 4:. CASPASE -3 activity assay in the three induced groups (CeO2 NPs: 10mg/ml, H. leucospilota extract: 20 mg/ml, combination CeO2 and H. leucospilota extract: 15 mg/ml) and control groups (albendazole as positive control group and PBS as negative control group)
Fig. 5:. After two months, mice infected with cysts in peritoneal cavity and liver (by injection of 2000 viable protoscoleces in /0.5mL sterile PBS)
Adibpour,
Antibacterial and Antifungal Activity of Holothuria leucospilota Isolated From Persian Gulf and Oman Sea.
2014, Pubmed,
Echinobase
Adibpour,
Antibacterial and Antifungal Activity of Holothuria leucospilota Isolated From Persian Gulf and Oman Sea.
2014,
Pubmed
,
Echinobase
Ahmadnia,
In vivo evaluation of the efficacy of albendazole sulfoxide and albendazole sulfoxide loaded solid lipid nanoparticles against hydatid cyst.
2013,
Pubmed
Andalib Aliabady,
A case report of muscle hydatidosis from iran.
2015,
Pubmed
Bordbar,
High-value components and bioactives from sea cucumbers for functional foods--a review.
2011,
Pubmed
,
Echinobase
Cai,
Dimeric drug polymeric nanoparticles with exceptionally high drug loading and quantitative loading efficiency.
2015,
Pubmed
Dahle,
Environmental geochemistry of cerium: applications and toxicology of cerium oxide nanoparticles.
2015,
Pubmed
Elmore,
Apoptosis: a review of programmed cell death.
2007,
Pubmed
Foroutan-Rad,
Holothuria leucospilota Extract Induces Apoptosis in Leishmania major Promastigotes.
2016,
Pubmed
,
Echinobase
Gangwar,
In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus.
2013,
Pubmed
Hu,
Drug-induced apoptosis of Echinococcus granulosus protoscoleces.
2011,
Pubmed
Jahani,
Improved serodiagnosis of hydatid cyst disease using gold nanoparticle labeled antigen B in naturally infected sheep.
2014,
Pubmed
Khademvatan,
Molecular characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran.
2013,
Pubmed
Khademvatan,
Cytotoxic Activity of Holothuria leucospilota Extract against Leishmania infantum In Vitro.
2016,
Pubmed
,
Echinobase
Khademvatan,
Echinococcus granulosus genotypes in Iran: a systematic review.
2019,
Pubmed
Khalkhali,
Prevalence of cystic echinococcosis in Iran: a systematic review and meta-analysis.
2018,
Pubmed
Labsi,
Antihydatic and immunomodulatory effects of Punica granatum peel aqueous extract in a murine model of echinococcosis.
2016,
Pubmed
Lashkarizadeh,
Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces.
2015,
Pubmed
Lawrence,
Bioactivity as an options value of sea cucumbers in the Egyptian Red Sea.
2010,
Pubmed
,
Echinobase
Li,
Review of the apoptosis pathways in pancreatic cancer and the anti-apoptotic effects of the novel sea cucumber compound, Frondoside A.
2008,
Pubmed
,
Echinobase
Ling,
Chemical synthesis and assembly of uniformly sized iron oxide nanoparticles for medical applications.
2015,
Pubmed
Lv,
Surgical treatment of multiple hydatid cysts in the liver of a pediatric patient.
2015,
Pubmed
Lötsch,
Intra-cystic concentrations of albendazole-sulphoxide in human cystic echinococcosis: a systematic review and analysis of individual patient data.
2016,
Pubmed
Maggiore,
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes.
2012,
Pubmed
Mahmoudvand,
Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts.
2014,
Pubmed
Mahmoudvand,
Protoscolecidal Effect of Berberis vulgaris Root Extract and Its Main Compound, Berberine in Cystic Echinococcosis.
2014,
Pubmed
Moazeni,
In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces.
2012,
Pubmed
Pakala,
Hepatic Echinococcal Cysts: A Review.
2016,
Pubmed
Pensel,
Cystic echinococcosis therapy: Albendazole-loaded lipid nanocapsules enhance the oral bioavailability and efficacy in experimentally infected mice.
2015,
Pubmed
Rubio,
Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system.
2016,
Pubmed
Sabzevari,
Traumatic rupture of liver hydatid cysts into the peritoneal cavity of an 11-year-old boy: a case report from Iran.
2017,
Pubmed
Sadjjadi,
In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces.
2008,
Pubmed
Spicher,
In vitro and in vivo effects of 2-methoxyestradiol, either alone or combined with albendazole, against Echinococcus metacestodes.
2008,
Pubmed
Spicher,
In vitro and in vivo treatments of echinococcus protoscoleces and metacestodes with artemisinin and artemisinin derivatives.
2008,
Pubmed
Spotin,
Genetic variability of Echinococcus granulosus complex in various geographical populations of Iran inferred by mitochondrial DNA sequences.
2017,
Pubmed
Urrea-París,
Relationship between the efficacy of praziquantel treatment and the cystic differentiation in vivo of Echinococcus granulosus metacestode.
2002,
Pubmed
Yousefi,
In vitro activity and cytotoxicity of Crocus sativus extract against leihmania major (MRHO/IR/75/ER).
2014,
Pubmed