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Genes Genomics
2019 Dec 01;4112:1397-1415. doi: 10.1007/s13258-019-00864-0.
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Transcriptome analysis to characterize the genes related to gonad growth and fatty acid metabolism in the sea urchin Strongylocentrotus intermedius.
Wang H
,
Ding J
,
Ding S
,
Chang Y
.
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BACKGROUND: Sea urchin gonads of both sexes, commonly termed "roe", are highly valued seafood delicacies, and Strongylocentrotus intermedius is considered one of the tastiest sea urchins. In order to produce high-quality gonads for consumption and clarify the mechanism of gonad growth and development of the sea urchin, more genetic information, especially at the transcriptome level, is needed.
OBJECTIVE: A more thorough understanding of sea urchin gonad growth and development in both sexes could enable regulation of these processes at several stages with the aim of suppressing gametogenesis in order to produce high-quality gonads for consumption.
METHODS: The adult sea urchins S. intermedius were cultured for 3 months, and were sampled for the gonadal transcriptome analysis which has been performed on the RNAs of three male and female adults of S. intermedius in each gonad development stage.
RESULTS: Illumina sequencing raw sequence data was deposited in the NCBI Sequence Read Archive (SRA) database (PRJNA532998). It generated 560,196,356 raw reads and 548,956,944 clean reads were acquired, which were assembled into 107,850 transcripts with 44,124 genes. Comparative analysis showed the differentially expressed genes (DEGs) from 114 to 2566. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to determine the functional significance of these DEGs. We have selected 9 genes related to growth and 12 genes related to fatty acid biosynthesis and metabolism in sea urchin gonads.
CONCLUSION: These data for sea urchins were intended to provide markers for gonad growth and development that can be accumulated for use in aquaculture applications.
2018YFD0901601 National Key Research and Development Program of China, 201814 Marine and Fishery Scientific Research Project of Liaoning Province, 2018-KF-18 Key Laboratory of Mariculture& Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, HDYJ201807 PhD research startup foundation of Dalian Ocean University, 31672652 National Natural Science Foundation of China
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