de Oliveira AP et al. (2019), Elemental imaging by Laser-Induced Breakdown Sp...

ECB-ART-47342

Sci Rep 2019 Jul 25;91:10827. doi: 10.1038/s41598-019-47338-7.

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Elemental imaging by Laser-Induced Breakdown Spectroscopy to evaluate selenium enrichment effects in edible mushrooms.

de Oliveira AP , de Oliveira Leme F , Nomura CS , Naozuka J .

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Mushrooms are bioaccumulating organisms commonly used in selenium (Se) enrichment studies. However, the addition of Se in the culture medium may alter the distribution of other essential elements in the mushroom fruiting body. To evaluate the effects of the Se enrichment, Ca, Mg, and K distributions in pink oyster (Pleurotus djamor) and K and Mg distributions in white oyster (Pleurotus ostreatus) mushrooms were mapped by laser-induced breakdown spectroscopy (LIBS), which can be used at room temperature and requires minimal or no sample preparation. It was verified that Se enrichment favoured the accumulation of Ca in the lower part of the pink oyster mushroom and prevented the transport of this element to the edges and tops. The Se enrichment also altered the distribution of K and Mg, decreasing the numerical correlation between the K and Mg distributions (R² = 0.5871). In the white oyster mushroom, however, despite the changes in the morphological characteristics of the fruiting bodies after enrichment, there were generally nonsignificant differences in the K and Mg distributions between the control and the Se-enriched mushrooms.

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References [+] :

Gimenez, 3D Imaging of Nanoparticle Distribution in Biological Tissue by Laser-Induced Breakdown Spectroscopy. 2016, Pubmed

	Figure 1. LIBS spectrum and individual signals for (A) carbon, (B) magnesium, (C) calcium, and (D) potassium of the pink oyster mushroom (accumulated signals from 10 laser shots).
	Figure 2. LIBS spectrum and individual signals for (A) carbon, (B) magnesium, and (C) potassium of the white oyster mushroom (accumulated signals from 10 laser shots).
	Figure 3. Fruiting body (a) and elemental distribution map of Ca (b), K (c), and Mg (d) in the control group, and fruiting body (e) and elemental distribution map of Ca (f), K (g), and Mg (h) in the Se-enriched pink oyster mushroom.
	Figure 4. Fruiting body (a) and elemental distribution map of K (b) and Mg (c) in the control group, and fruiting body (d) and elemental distribution map of K (e) and Mg (f) in the Se-enriched white oyster mushroom.
	Figure 5. Elemental distribution map of C in the control (a) and Se-enriched (b) pink oyster mushroom, and control (c) and Se-enriched (d) white oyster mushroom.
	Figure 6. Fruiting body (a) and elemental distribution map of Ca (b), K (c), and Mg (d) in the control group, and fruiting body (e) and elemental distribution map of Ca (f), K (g), and Mg (h) in the Se-enriched pink oyster mushroom using C(I)247.856 nm as an internal standard.
	Figure 7. Fruiting body (a) and elemental distribution map of K (b) and Mg (c) in the control group, and fruiting body (d) and elemental distribution map of K (e) and Mg (f) in the Se-enriched white oyster mushroom using C(I)247.856 nm as an internal standard.