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Nectria-related fungi causing dieback and canker diseases in China, with Neothyronectriacitri sp. nov. described.
Yang Q
,
Chen WY
,
Jiang N
,
Tian CM
.
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To clarify phylogenetic relationships amongst Nectria, Neothyronectria and Thyronectria in Nectriaceae, we examined detailed morphological characters and performed phylogenetic analyses of a concatenated dataset, based on the ITS, LSU, tef1 and tub2 DNA sequences of fungal specimens in China. Four species of nectria-related fungi were identified, i.e. Nectriadematiosa, N.pseudotrichia, Neothyronectriacitri and Thyronectriapinicola. The newly described species, Neothyronectriacitri, is characterised by its ascomatal wall with bright yellow scurf, unitunicate asci, each with 4-spored and ascospores allantoid to short-cylindrical, uniseriate, muriform, hyaline to slightly yellowish-brown. This species has affinities with other one known species of Neothyronectria and can be distinguished by molecular data.
Figure 1. Maximum parsimony phylogenetic tree generated from analysis of a combined ITS, LSU, tef1 and tub2 sequence dataset for 59 taxa of Allantonectria, Nectria, Neothyronectria and Thyronectria. Emericellopsisglabra (CBS 125295), Hydropisphaerafungicola (CSB 122304), Nectriopsisexigua (CBS 126110) and Verrucostomafreycinetiae (MAFF 240100) as outgroup taxa. Values above the branches indicate maximum parsimony and maximum likelihood bootstrap (left, MP BP ≥ 50%; right, ML BP ≥ 50%). The branches with significant BIPP values (≥ 0.95) in the BI analysis are thickened. Scale bar = 80 nucleotide substitutions. Strains in current study are in blue. Ex-type strains are indicated in bold.
Figure 2. Nectriadematiosa (CFCC 53585) A–B habit of conidiomata on branches C transverse section of conidioma D longitudinal section of conidioma E conidiophores F–G conidia. Scale bars: 1 mm (A–C); 500 μm (D); 10 μm (E–G).
Figure 3. Nectriapseudotrichia (CFCC 53587) A–B habit of conidiomata on branches C–D conidiophores E–F conidia. Scale bars: 1 mm (A–B); 10 μm (C–F).
Figure 4. Neothyronectriacitri (CFCC 53590) A–B habit of conidiomata on branches C transverse section of conidioma D longitudinal section of conidioma E–F asci G–H ascospores. Scale bars: 500 μm (B–D); 10 μm (E–H).
Figure 5. Thyronectriapinicola (CFCC 53593) A–C habit of conidiomata on branches D longitudinal section of conidioma E–G conidiogenous cells with conidia H conidia I–J culture on PDA and conidiomata. Scale bars: 1 mm (B); 500 μm (C–D); 10 μm (E–H).
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