Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Clin Lab Anal
2019 May 01;334:e22830. doi: 10.1002/jcla.22830.
Show Gene links
Show Anatomy links
Determination of vitamin D in tears of healthy individuals by the electrochemiluminescence method.
Lai YT
,
Cerquinho RG
,
Perez MM
,
Alves BDCA
,
Pereira EC
,
Azzalis LA
,
Junqueira VBC
,
Soares LR
,
Fonseca FLA
.
???displayArticle.abstract???
BACKGROUND: Vitamin D is a fat-soluble steroid hormone which can be converted into various forms and is of extreme physiological importance to our body. However, its functions and local metabolic pathways in some organs, such as the eye, have not yet been well studied. We aimed to verify the correlation between vitamin D levels in blood and tear fluid and the possibility of using tear fluid as a biological material for monitoring eye disorders in the future.
METHODS: The electrochemiluminescence method was used to examine blood and tear samples collected with Schirmer test strips from 21 individuals without ocular disease.
RESULTS: At the 95% confidence interval, mean tear fluid vitamin D = 37.8 ± 3.6 ng/mL, which is higher than the serum level, with a mean of 30.3 ± 7.7 ng/mL; Lin''s concordance correlation coefficient = -0.018 (-0.174; 0.139), Pearson''s coefficient = -0.070, and the Bland-Altman coefficient = -11.12 (-30.40; 8.16). Results were obtained using the program Stata version 11.0.
CONCLUSION: It is possible to determine vitamin D levels in tear fluid using the electrochemiluminescence method, and as the results do not correlate with blood, there is possibility of using tear fluid as a biological matrix for detection of vitamin D, which may increase the possibilities of new studies in eye disorders.
,
The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop (2007).
2007, Pubmed
,
The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop (2007).
2007,
Pubmed
Alsalem,
Characterization of vitamin D production by human ocular barrier cells.
2014,
Pubmed
Bland,
Regulation of vitamin D-1alpha-hydroxylase in a human cortical collecting duct cell line.
2001,
Pubmed
Elizondo,
Effect of vitamin D receptor knockout on cornea epithelium wound healing and tight junctions.
2014,
Pubmed
Enko,
25-hydroxy-Vitamin D status: limitations in comparison and clinical interpretation of serum-levels across different assay methods.
2014,
Pubmed
Hansdottir,
Respiratory epithelial cells convert inactive vitamin D to its active form: potential effects on host defense.
2008,
Pubmed
Heo,
Features of the retinal environment which affect the activities and product profile of cholesterol-metabolizing cytochromes P450 CYP27A1 and CYP11A1.
2012,
Pubmed
Hertting,
Vitamin D induction of the human antimicrobial Peptide cathelicidin in the urinary bladder.
2010,
Pubmed
Holick,
Vitamin D deficiency.
2007,
Pubmed
Jee,
Serum 25-Hydroxyvitamin D Levels and Dry Eye Syndrome: Differential Effects of Vitamin D on Ocular Diseases.
2016,
Pubmed
Jeffery,
1,25-Dihydroxyvitamin D3 and IL-2 combine to inhibit T cell production of inflammatory cytokines and promote development of regulatory T cells expressing CTLA-4 and FoxP3.
2009,
Pubmed
Kemmis,
Mammary epithelial cell transformation is associated with deregulation of the vitamin D pathway.
2008,
Pubmed
Lai,
Determination of vitamin D in tears of healthy individuals by the electrochemiluminescence method.
2019,
Pubmed
,
Echinobase
Lin,
Enhancement of vitamin D metabolites in the eye following vitamin D3 supplementation and UV-B irradiation.
2012,
Pubmed
Lu,
Vitamin D in Tear Fluid.
2015,
Pubmed
Matsuoka,
Suntanning and cutaneous synthesis of vitamin D3.
1990,
Pubmed
Parikh,
Vitamin D resistance in chronic kidney disease (CKD).
2014,
Pubmed
Sethu,
Correlation between tear fluid and serum vitamin D levels.
2016,
Pubmed
Yildirim,
Dry eye in vitamin D deficiency: more than an incidental association.
2016,
Pubmed
Yoon,
Low Serum 25-Hydroxyvitamin D Levels Are Associated with Dry Eye Syndrome.
2016,
Pubmed