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Development
2017 Apr 01;1447:1201-1210. doi: 10.1242/dev.144170.
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Transient translational quiescence in primordial germ cells.
Oulhen N
,
Swartz SZ
,
Laird J
,
Mascaro A
,
Wessel GM
.
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Stem cells in animals often exhibit a slow cell cycle and/or low transcriptional activity referred to as quiescence. Here, we report that the translational activity in the primordial germ cells (PGCs) of the sea urchin embryo (Strongylocentrotus purpuratus) is quiescent. We measured new protein synthesis with O-propargyl-puromycin and L-homopropargylglycine Click-iT technologies, and determined that these cells synthesize protein at only 6% the level of their adjacent somatic cells. Knockdown of translation of the RNA-binding protein Nanos2 by morpholino antisense oligonucleotides, or knockout of the Nanos2 gene by CRISPR/Cas9 resulted in a significant, but partial, increase (47%) in general translation specifically in the PGCs. We found that the mRNA of the translation factor eEF1A is excluded from the PGCs in a Nanos2-dependent manner, a consequence of a Nanos/Pumilio response element (PRE) in its 3''UTR. In addition to eEF1A, the cytoplasmic pH of the PGCs appears to repress translation and simply increasing the pH also significantly restores translation selectively in the PGCs. We conclude that the PGCs of this sea urchin institute parallel pathways to quiesce translation thoroughly but transiently.
Ahringer,
Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3' untranslated region.
1991, Pubmed
Ahringer,
Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3' untranslated region.
1991,
Pubmed
Asaoka-Taguchi,
Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos.
1999,
Pubmed
Ben-Tabou de-Leon,
Gene regulatory control in the sea urchin aboral ectoderm: spatial initiation, signaling inputs, and cell fate lockdown.
2013,
Pubmed
,
Echinobase
Boonsanay,
Regulation of Skeletal Muscle Stem Cell Quiescence by Suv4-20h1-Dependent Facultative Heterochromatin Formation.
2016,
Pubmed
Brandhorst,
Two-dimensional gel patterns of protein synthesis before and after fertilization of sea urchin eggs.
1976,
Pubmed
,
Echinobase
Cheers,
Rapid microinjection of fertilized eggs.
2004,
Pubmed
,
Echinobase
Cheung,
Molecular regulation of stem cell quiescence.
2013,
Pubmed
Cho,
Cap-dependent translational inhibition establishes two opposing morphogen gradients in Drosophila embryos.
2006,
Pubmed
Cormier,
eIF4E association with 4E-BP decreases rapidly following fertilization in sea urchin.
2001,
Pubmed
,
Echinobase
Dalby,
Discrete sequence elements control posterior pole accumulation and translational repression of maternal cyclin B RNA in Drosophila.
1993,
Pubmed
Epel,
Protein synthesis in sea urchin eggs: a "late" response to fertilization.
1967,
Pubmed
,
Echinobase
Fukada,
Molecular signature of quiescent satellite cells in adult skeletal muscle.
2007,
Pubmed
Gilboa,
Repression of primordial germ cell differentiation parallels germ line stem cell maintenance.
2004,
Pubmed
Goldstein,
Home sweet home: skin stem cell niches.
2012,
Pubmed
Hayashi,
Nanos suppresses somatic cell fate in Drosophila germ line.
2004,
Pubmed
Irish,
The Drosophila posterior-group gene nanos functions by repressing hunchback activity.
1989,
Pubmed
Juliano,
Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Kadyrova,
Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline.
2007,
Pubmed
Karp,
3H-amino acid uptake and incorporation in sea urchin gastrulae and exogastrulae: an autoradiographic study.
1975,
Pubmed
,
Echinobase
Lai,
Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells.
2012,
Pubmed
Lai,
Nanos1 functions as a translational repressor in the Xenopus germline.
2011,
Pubmed
López-Iglesias,
Hypoxia induces pluripotency in primordial germ cells by HIF1α stabilization and Oct4 deregulation.
2015,
Pubmed
Morales,
Translational control genes in the sea urchin genome.
2006,
Pubmed
,
Echinobase
Morris,
Capturing and profiling adult hair follicle stem cells.
2004,
Pubmed
Murata,
Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
1995,
Pubmed
Nakamura-Ishizu,
The analysis, roles and regulation of quiescence in hematopoietic stem cells.
2014,
Pubmed
Oulhen,
Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo.
2016,
Pubmed
,
Echinobase
Oulhen,
The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.
2013,
Pubmed
,
Echinobase
Pattabiraman,
Tackling the cancer stem cells - what challenges do they pose?
2014,
Pubmed
Rezza,
Adult stem cell niches: cellular and molecular components.
2014,
Pubmed
Richmond,
Dormant Intestinal Stem Cells Are Regulated by PTEN and Nutritional Status.
2015,
Pubmed
Richmond,
Factors regulating quiescent stem cells: insights from the intestine and other self-renewing tissues.
2016,
Pubmed
Sato,
Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos.
2007,
Pubmed
Sonoda,
Recruitment of Nanos to hunchback mRNA by Pumilio.
1999,
Pubmed
Starck,
A general approach to detect protein expression in vivo using fluorescent puromycin conjugates.
2004,
Pubmed
Swartz,
Deadenylase depletion protects inherited mRNAs in primordial germ cells.
2014,
Pubmed
,
Echinobase
Tanaka,
Study of the Lineage and Cell Cycle of Small Micromeres in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus: (small micromeres/cell cycle/cell lineage/unequal cleavage/sea urchin).
1990,
Pubmed
,
Echinobase
Valcourt,
Staying alive: metabolic adaptations to quiescence.
2012,
Pubmed
Voronina,
Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development.
2008,
Pubmed
,
Echinobase
Webb,
FOXO3 shares common targets with ASCL1 genome-wide and inhibits ASCL1-dependent neurogenesis.
2013,
Pubmed
Wessel,
The biology of the germ line in echinoderms.
2014,
Pubmed
,
Echinobase
Wharton,
RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos.
1991,
Pubmed
Williamson,
Germ cell development in Drosophila.
1996,
Pubmed
Wreden,
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.
1997,
Pubmed
Zhang,
A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line.
1997,
Pubmed
